ESTIMATION OF HETEROZYGOSITY FOR SINGLE PROBE, MULTIL. DNA FINGER.

可以从人口中得出的最有用的参数之一 遗传数据是平均杂合度(H)，传统上 被用来对比不同种群之间的遗传变异水平 比较不同基因座之间的遗传变异。尽管有 DNA指纹技术越来越多地应用于自然种群和 对于人类的遗传鉴定，显式的估计方法 H的DNA指纹数据还没有开发出来。贡献 这种遗漏的原因是无法确定相对重要的 多位点指纹探针的遗传信息，如 基因座的数量、等位基因的数量以及这些基因的分布 将等位基因转化为特定的基因座。我们得到了显式的计算结果 期望的平均杂合度和最大值 估计一下。这些估计是基于DNA限制模式 用于人类指纹研究的典型矩阵和 自然种群。对于我们实验室的几个经验数据集， 这些预估数据显示彼此比较接近。这 观察和考虑缺失等位基因和 不同数量的基因座表明，平均杂合度可以是 使用表型DNA指纹模式进行了精确的近似，因为 这个参数对某些基因的缺失相对不敏感。 信息。

One of the most useful parameters that can be derived from population genetic data is the average heterozygosity (H), which has traditionally been employed to contrast levels of genetic variation among populations and to compare genetic variation among different loci. In spite of the increasing application of DNA fingerprinting to natural populations and to the genetic identification of humans, explicit methods for estimation of H from DNA fingerprinting data have not been developed. Contributing to this omission is the inability to determine relatively important genetic information for multilocus fingerprinting probes, such as the number of loci, the number of alleles, and the distribution of these alleles into specific loci. We have derived explicit calculations for the expected average heterozygosity and a maximum value for this estimate. These estimates are based upon the DNA restriction pattern matrices that are typical for fingerprinting studies of humans and natural populations. For several empirical datasets from our laboratory, these estimates are shown to be relatively close to each other. This observation, and consideration of the effect of missing alleles and alternate numbers of loci, suggest that the average heterozygosity can be accurately approximated using phenotypic DNA fingerprint patterns because this parameter is relatively insensitive to the lack of certain genetic information.