CHARACTERIZATION OF DROSOPHILA MELANOGASTER--ETS AND ETS-LIKE GENES

三个果蝇基因与ETS相关的细胞序列 鸡反转录病毒E26区已在本地区分离 实验室。最相关基因D-ETS的特征部分， 与鸡c-ets-1的最后两个外显子有90%以上的同源性 基因在预测的氨基酸水平。D-ETS(基因组)的区域 克隆)5‘到该区域已经被证明存在于一个cDNA克隆中， 该序列与鸡c-ETS-1基因不同。D-ETS是 在整个发育过程中表达为4.7kb的转录本。它是本地化的 位于染色体58A/B的D-ELG(果蝇ETS样基因) 定位于染色体上97D位置的相关基因 染色体3R。这个基因是由从蚕蛹中分离出来的克隆来表示的 C DNA文库。其序列与D-ETS有60%的氨基酸相似性。 D-ELG被表达为一个2.0kb的转录本 在整个胚胎发育过程中，以及蛹和成虫阶段，但没有 从幼虫身上。D-ELG在所有胚胎细胞中均有表达 转录激活，没有区域定位。三分之一 通过与v-ETS的杂交鉴定出E13B基因，并对其基因组进行了分析 克隆已经被部分测序。与E13B杂交的cDNA克隆 显示了一条长长的Poly A+尾巴，也正在进行测序。E13B基因是 仅在蛹和成虫中表达为1.6kb的RNA物种。它是 位于染色体3L的66A位。

Three Drosophila genes with cellular sequences related to the ets region of the chicken retrovirus, E26, have been isolated in this laboratory. The characterized portion of the most related gene, D-ets, shows over 90% identity with the last two exons of the chicken c-ets-1 gene at the predicted amino acid level. The region of D-ets (a genomic clone) 5' to this region has been shown to be present in a cDNA clone, and this sequence is divergent from the chicken c-ets-1 gene. D-ets is expressed as a 4.7 kb transcript throughout development. It is localized to chromosome 3R at position 58A/B. D-elg (Drosophila ets-like gene) is a related gene that has been localized to chromosomal position 97D on chromosome 3R. This gene is represented by a clone isolated from a pupae cDNA library. Its sequence shows 60% amino acid similarity with D-ets. D-elg is expressed as a single transcript of 2.0 kb that is expressed throughout embryogenesis, as well as pupae and adult stages, but absent from larvae. D-elg is expressed in all embryonic cells prior to transcriptional activation and has no regional localization. A third gene, E13B, was identified by hybridization with v-ets, and a genomic clone has been partially sequenced. A cDNA clone that hybridized to E13B shows a long Poly A+ tail and is also being sequenced. The E13B gene is expressed as a 1.6 kb RNA species in pupae and adults only. It is located on chromosome 3L at position 66A.