课题基金 / 基金详情

DEVELOPMENT OF RAMAN SPECTROSCOPY FOR ENZYME-SUBSTRATE REACTIONS

DEVELOPMENT OF RAMAN SPECTROSCOPY FOR ENZYME-SUBSTRATE REACTIONS
酶-底物反应拉曼光谱的发展
批准号：
3858004
负责人：
R L BERGER
金额：
$0.0万
依托单位：
NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3858004
关键词：
Raman spectrometry adenosine diphosphate adenosine triphosphate bioengineering /biomedical engineering chemical reaction enzyme mechanism enzyme substrate analog enzyme substrate complex infrared spectrometry magnesium time resolved data

项目摘要

项目成果

R L BERGER的其他基金

相似基金

相关文献

中文摘要
翻译
在桑迪亚国家实验室获得了ATP和ADP的拉曼光谱 在新墨西哥大学。它们提供了基线光谱，因为我们 探索获取三磷酸腺苷和各种三磷酸腺苷光谱的可行性 使用时间分辨拉曼光谱的络合物和类似物。我们有 表明拉曼差谱提供了所需的灵敏度 两个相对稀薄的样品中的小扰动，如ATP 与镁的络合物以及与ADP的比较。不同之处在于 在很高的磷酸盐浓度下进行的红外光谱分析 有时会与其他ATP或ADP分子发生络合 解释起来很困难。
英文摘要
Raman Spectra of ATP and ADP were obtained at Sandia National Laboratories and at the University of New Mexico. These provide baseline spectra as we explore the feasibility of obtaining spectra of ATP and various ATP complexes and analogs using time resolved Raman Spectroscopy. We have shown that Raman difference spectra provide the sensitivity required for small perturbations in two relatively dilute samples such as the ATP complex with Mg and in comparison to ADP. The differences were known from infra-red spectroscopy which is done at very high phosphate concentration where complexing with other ATP or ADP molecules sometimes occurs making interpretation difficult.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
DEVELOPMENT OF BIOPHYSICAL METHODS FOR STUDY OF BIO-MACROMOLECULAR REACTIONS
DEVELOPMENT OF BIOCALORIMETERS FOR SOLUTION AND CELL BIOCHEMICAL STUDIES
DEVELOPMENT OF NIR FOR MONITORING ENZYME-SUBSTRATE REACTIONS
DEVELOPMENT OF BIOPHYSICAL METHODS FOR STUDY OF BIO-MOLECULAR REACTIONS
海外基金