课题基金 / 基金详情

DEVELOPMENT OF NIR FOR MONITORING ENZYME-SUBSTRATE REACTIONS

DEVELOPMENT OF NIR FOR MONITORING ENZYME-SUBSTRATE REACTIONS
开发用于监测酶-底物反应的近红外光谱
批准号：
3858005
负责人：
R L BERGER
金额：
$0.0万
依托单位：
NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3858005
关键词：
adenosine diphosphate adenosine triphosphate bioengineering /biomedical engineering chemical reaction enzyme mechanism enzyme substrate complex infrared spectrometry phosphates

项目摘要

项目成果

R L BERGER的其他基金

相似基金

相关文献

中文摘要
翻译
我们已经使用了近红外光谱来量化每一种 化合物三磷酸腺苷、二磷酸腺苷、磷酸三氢叶酸。我们成功地制作了校正曲线 对于每个在1到25微摩尔范围内的。最近，三者的混合物 磷酸盐化合物也在相同的浓度范围内进行了定量。 因此，可以利用NEAR来区分ATP、ADP和P(I 红外线。因为在近红外光谱中有显著的变化 ATP和ADP的光谱以及ATP和ADP之间的主要变化 在磷酸盐部分中，随之而来的是差异 在近红外光谱中，ADP和ATP都在这种环境中。
英文摘要
We have employed near infrared spectroscopy to quantify each of the compounds ATP,ADP, and PO 4. We have successfully made calibration curves for each in the 1 to 25 micromolar regime. Recently, mixtures of the three phosphate compounds were also quantified in the same concentration region. Hence, the distinction of ATP, ADP, and P(i) can be achieved with near infrared. Because there are significant variations in the near infrared spectra of ATP and ADP and the majority of change between ATP and ADP lies within the phosphate moiety, it follows that the differences being followed in the near infrared spectra of ADP and ATP lie within this environment.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
DEVELOPMENT OF NIR FOR MONITORING ENZYME-SUBSTRATE REACTIONS
DEVELOPMENT OF BIOPHYSICAL METHODS FOR STUDYING BIOCHEMICAL REACTIONS
DEVELOPMENT OF BIOPHYSICAL METHODS FOR STUDY OF BIO-MOLECULAR REACTIONS
DEVELOPMENT OF BIOCALORIMETERS FOR SOLUTION AND CELL BIOCHEMICAL STUDIES
海外基金