A RESOURCE FOR CONSTRUCTION OF 2D GEL PROTEIN DATABASES

在冷泉的QUEST资源设施的长期目标 Harbor实验室将开发2D凝胶蛋白质数据库作为强大的工具 用于生物学研究。 具体而言，该设施旨在1）进一步 发展了蛋白质二维分析的计算机技术 凝胶，2）在三个重要的研究中建立模型蛋白质数据库 3）以其他人无法理解的形式传播这些数据库。 研究人员可以在个人电脑上使用。 QUEST资源实际上是两个资源合二为一。 在计算机方面， 软件开发人员将寻求使2D凝胶图像分析几乎 完全自动化，他们将寻求提高生产力的 通过改进用户界面建立蛋白质数据库的科学家， 数据库管理工具。 在实验室方面， QUEST设施将进行核心和协作实验， 蛋白质数据库，并确定大量的蛋白质检测 在二维凝胶上。 将为以下研究领域开发数据库：1）细胞- 哺乳动物细胞中的增殖，2）核基因调节蛋白，和 3)酵母细胞和分子生物学。 每个蛋白质数据库都是建立在 以前在QUEST设施和科学研究方面的经验 核心员工和协作员工。 QUEST实验室将包括一个蛋白质化学设施， 身份证明 将常规使用微测序来识别 蛋白质从二维凝胶。 抗体将被收集和使用 来鉴定当前感兴趣的蛋白质。 肽谱和氨基酸 组合物将提供额外的信息， 来自不同物种和不同实验室的地图。 QUEST蛋白质数据库将分发给其他研究人员， 适用于Macintosh计算机的CD-ROM磁盘。 凝胶图像，定量 将为每个人提供数据、注释和实验室记录。 实验 这些数据将与Hypercard和许多 用于统计分析和数据显示的软件包， 现在可以在Macintosh上使用。 作为研究正常和疾病相关蛋白质谱的技术 2D凝胶蛋白质数据库将同时包含细胞和体液， 对健康问题直接和长期影响。 已经标记 已经发现了白血病等疾病的蛋白质。 术语，自动化2D凝胶分析，加上广泛的蛋白质数据库， 不仅要根据标记蛋白， 蛋白质谱，包括遗传多态性、改变的调节 蛋白质（致癌基因产物），以及对外部因子（病毒， 压力、毒品）。

The long-term objective of the QUEST resource facility at the Cold Spring Harbor Laboratory is to develop 2D gel protein databases as powerful tools for biological research. Specifically, this facility seeks 1) to further develop the computer technology for analysis of two-dimensional protein gels, 2) to build model protein databases in three important research areas, and 3) to disseminate these databases in a form that other researchers can use on personal computers. The QUEST resource is really two resources in one. On the computer side, the software developers will seek to make 2D gel image analysis almost totally automatic, and they will seek to improve the productivity of the scientists who build protein databases by improving the user interface and the database management tools. On the laboratory side, the members of the QUEST facility will perform core and collaborative experiments to build protein databases and to identify large numbers of the proteins detected on two dimensional gels. Databases will be developed for the following research areas: 1) cell- proliferation in mammalian cells, 2) nuclear gene-regulatory proteins, and 3) yeast cell and molecular biology. Each protein database builds on previous experience at the QUEST facility and on the scientific research of core and collaborative workers. The QUEST laboratory will include a protein chemistry facility for protein identifications. Microsequencing will be used routinely to identify proteins from two-dimensional gels. Antibodies will be collected and used to identify proteins of current interest. Peptide profiles and amino acid compositions will provide additional information to relate the protein maps from different species and from different laboratories. The QUEST protein databases will be disseminated to other researchers on CD-ROM disks for use on Macintosh computers. Gel images, quantitative data, annotations, and laboratory records will be provided for each experiment. The data will be compatible with Hypercard and numerous software packages for statistical analysis and data presentation that are now available on the Macintosh. As a technology to study the normal and disease-related protein profiles of cells and body fluids, 2D gel protein databases will have both immediate and long-term impact on health problems. Already marker proteins for diseases such as leukemias have been found. In the long term, automated 2D gel analysis, coupled with extensive protein databases, should give diagnoses based not just on marker proteins but on the entire protein profile including genetic polymorphisms, altered regulatory proteins (oncogene products), and responses to external agents (viruses, stress, drugs).