PHE-MET-ARG-PHE-NH2 LIKE PEPTIDES IN CNS--FUNCTION AND DISTRIBUTION

神经肽FLIPQRF-NH（F-8-F-NH 2）最初分离自 牛脑，并发现具有吗啡调节活性。 F-8-F-NH 2是 高度定位于垂体后叶的神经末梢。 的来源 这种垂体F-8-F-NH 2通过各种损伤来研究。 我们的结果 这表明，至少部分垂体F-8-F-NH 2来源于 视上核，在那里它可以与Arg 8-加压素共定位。 的 F-8-F-NH 2在吗啡耐受和失败镇痛中的作用 以F-8-F-NH_2抗血清IgG为拮抗剂。 注射（ICV） 从F-8-F-NH 2抗血清中提取的IgG可恢复对吗啡的镇痛反应 表明内源性F-8-F-NH 2有助于 对吗啡的耐受性 注射（ICV）F-8-F-NH 2可减弱 诱导镇痛在从属小鼠在激动的遭遇。 相比之下，ICV给予来自F-8-F-NH 2抗血清的IgG增加了 失败引起的镇痛水平。 这些结果提供了进一步 F-8-F-NH 2可能对内源性阿片样物质具有调节作用的证据 介导镇痛 在大鼠CNS中研究了F-8-F-NH 2的发育。 在18日之前，在脑或脊髓中未检测到F-8-F-NH 2 胚胎日 第一批F-8-F-NH 2阳性纤维和终末为 在胚胎第20天的正中隆起中检测到。 出生后，F- 8-F-NH_2系统在脑和脊髓中均迅速发育， 4周大的时候就像成年人一样。 F-8-F-NH_2体系的后期出现 在个体发育过程中，表明这些肽表达于 相对成熟的神经元 这些结果进一步表明，F-8-F-NH 2可能 在神经系统的发育中没有一般功能，但 而可能作为神经调质或神经递质，因为它的生产 始于神经元。

The neuropeptide FLFQPQRF-NH(F-8-F-NH2) was originally isolated from the bovine brain and found to have morphine modulating activity. F-8-F-NH2 is highly localized in nerve terminals in posterior pituitary. The source of this pituitary F-8-F-NH2 was studied by various lesions. Our results suggest that at least part of the pituitary F-8-F-NH2 originates from supraoptic nuclei where it may be co-localized with Arg8-vasopressin. The role of F-8-F-NH2 in morphine tolerance and defeat-induced analgesia was studied using IgG from F-8-F-NH2 antiserum as antagonist. Injection (ICV) of IgG from F-8-F-NH2 antiserum restored the analgesic response to morphine in morphine-tolerant rats suggesting that endogenous F-8-F-NH2 contributes to morphine tolerance. Injection (ICV) of F-8-F-NH2 attenuated the defeat induced analgesia in the subordinate mice during the agonistic encounter. In contrast, ICV administration of IgG from F-8-F-NH2 antiserum augmented the levels of defeat induced analgesia. These results provide further evidence that F-8-F-NH2 may have a modulatory role in endogenous opioid mediated analgesia. Development of F-8-F-NH2 was studied in the rat CNS. F-8-F-NH2 was not detected in the brain or the spinal cord before the 18th embryonic day. The first F-8-F-NH2 positive fibers and terminals were detected in the median eminence on the 20th embryonic day. Postnatally, F- 8-F-NH2 system developed rapidly both in the brain and spinal cord, and was adult like by the age of 4 weeks. The late appearance of F-8-F-NH2 system during the ontogeny suggests that these peptides are expressed in relatively mature neurons. These results further imply that F-8-F-NH2 may not have a general function in the development of the nervous system, but rather may act as a neuromodulator or neurotransmitter since its production starts in neurons.