A STUDY OF ADENOSINE RECEPTORS--ISOLATION AND CHARACTERIZATION

腺苷受体的纯化和性质研究进展 继续。使用我们拥有的一种新型亲和层析系统 此前开发的A1腺苷受体已成功纯化为 同质性，不仅来自大鼠脑膜，也来自大鼠睾丸 膜和人脑的膜。结果表明，分子量比表面积小。 这些纯化的受体中有相似的(34,000-41,000)，所有这些 受体是糖基化的。这三株A1的免疫学特性 用多克隆的蛋白质印迹比较腺苷受体。 纯化的大鼠脑A1腺苷受体抗体。 腺苷A1受体分布的免疫细胞化学研究 同时取大鼠脑组织作病理切片。在脑内观察到离散的斑点。 大脑皮层，视上核和室旁核，海马体， 小脑的浦肯野细胞、红核和中缝大核。

Purification and characterization of adenosine receptors have been continued. Using a novel affinity chromatography system which we had previously developed, A1 adenosine receptors were successfully purified to homogeneity, not only from rat brain membranes but also from rat testicular membranes and human brain membranes. It was shown that the molecular weight of these purified receptors were similar (34,000-41,000) and that all these receptors are glycosylated. Immunological properties among these three A1 adenosine receptors were compared by a western blot using a polyclonal antibody against the purified rat brain A1 adenosine receptor. Immunocytochemical studies of the distribution of A1 adenosine receptors in the rat brain were also performed. Discrete puncta were observed in the cerebral cortex, supraoptic and paraventricular nuclei, hippocampus, purkinje cells of the cerebellum, red nucleus and raphe magnus.