MULTISTATION COMPUTERIZED MOTION ANALYSIS SYSTEM

需要为多用户计算机化运动分析系统提供资金。 该系统由四部分组成：图像处理和图形分析 通过以太网链接到中央处理器和文件服务器的工作站， 太阳微系统公司SPARCstant330。软件将是2-D ExpertVision 用于分析细胞和粒子运动的系统(EV)，以及动态 用于分析细胞形状变化的形态系统(DMS)(来自 运动分析系统公司)。一台蔡司Axiovert 35显微镜也 需要高分辨率差分图像对比度(DIC)、相位、 暗场和荧光图像。 它的主要应用将是分析(I)趋光性信号 嗜盐杆菌和莱茵衣藻中的视紫红质(II) 利用耐光性蛋白在大肠杆菌趋化性中的激发信号 笼式化学效应器，(III)趋化性和细胞形状的变化 盘基网眼菌，(Iv)细菌和鱿鱼的膜马达 轴突，以及(V)完整和通透性草履虫的游泳行为。 主要用户群包括Spudich博士、Condeelis博士、Khan博士、Satir博士、 还有西格尔。所请求的硬件和软件将结合现有的 将有限能力的组件从我们的单个实验室转化为 近两个数量级的多站运动分析网络 更快的计算速度。

Funds are requested for a multiuser computerized motion analysis system. The system consists of four image processing and graphics analysis workstations linked via ethernet to a central processor and file server, a Sun Microsystems SPARCstation330. Software will be the 2-D ExpertVision System (EV) for the analysis of cell and particle motion, and the Dynamic Morphology System (DMS) for the analysis of cell shape changes (both from Motion Analysis Systems, Inc.). One Zeiss Axiovert 35 microscope is also requested for highly resolved differential image contrast (DIC), phase, darkfield, and fluorescence images. The main applications will be to analyze (i) phototaxis signaling by rhodopsins in Halobacterium halobium and Chlamydomonas reinhardtii, (ii) excitation signaling in chemotaxis of Escherichia coli using photolabile caged chemoeffectors, (iii) chemotaxis and cell shape changes in Dictyostelium discoideum, (iv) membrane motors in bacteria and in squid axons, and (v) swimming behavior of intact and permeabilized paramecia. The major user group is comprised of Drs. Spudich, Condeelis, Khan, Satir, and Segall. The requested hardware and software will incorporate existing components of limited capability from our individual laboratories into a multistation motion analysis network with nearly two orders of magnitude greater computational speed.