DEVELOPMENT OF IN VITRO PROPAGATED F344/N MONONUCLEAR CELL LINES

中国人单核细胞白血病(MNCL)的高背景发病率 费舍尔344只大鼠(20%到30%)混淆了这种评估和解释 两年内MNCL可能与化学治疗相关的发病率 慢性毒理学和致癌研究。一种F344大鼠白血病 移植模型已经被开发出来，用来描述这种情况的生物学特征 并对啮齿动物白血病和肿瘤生物学进行研究，确定其化学成分 治疗影响疾病表现。试管苗的开发与应用 增殖的F344/N单核白血病细胞将增强：(1) 诊断MNCL特异性单抗的研制 疾病的目的和分期。(2)使用目前可用的 大鼠细胞表面抗原和受体数据到已知的细胞化学， 形态和细胞生化数据及细胞毒性的测定 白血病细胞来源和功能谱系，以及(3)体外使用 测试以确定化学品的毒性和致癌性 MNCL体内移植模型的研究。 到目前为止，已经开发出几个杂交瘤细胞系，这些杂交瘤细胞系 被确定为分泌可能是这些白血病特有的抗体 细胞。细胞分离已经被开发出来，它允许分离 不同的白血病细胞类型。这些不同的白血病细胞亚群 已经通过几种途径在体内繁殖，并已经 冷冻保存以供将来鉴定，(包括单抗 分析)。以体外培养为扩散腔的方法， 软琼脂，和Dexter类型的培养和培养条件都得到了精炼。

The high background incidence of mononuclear cell leukemia (MNCL) in Fischer 344 rats (20 to 30%) confounds the evaluation and interpretation of possible chemical treatment related incidence of MNCL in two-year chronic toxicology and carcinogenesis studies. A F344 rat leukemia transplant model has been developed to characterize the biology of this rodent leukemia and to study the tumor biology and determine how chemical treatment effects disease expression. The development and use of in vitro propagated F344/N mononuclear leukemic cells will enhance: (1) development of monoclonal antibodies unique to MNCL for diagnostic purposes and staging of the disease. (2) the use of currently available rat cell surface antigen and receptor data to known cytochemical, morphological and cell biochemistry data and the determination of leukemic cell origin and functional lineage, and (3) the use of in vitro tests to determine the toxicity and carcinogenicity of chemicals under study in the in vivo MNCL transplant model. To date several hybridoma cell lines have been developed that have been determined to secrete antibodies that may be unique to these leukemic cells. Cell separations have been developed that allow separation of different leukemia cell types. These different subsets of leukemia cells have been propagated in vivo through several passages and have been cryopreserved for future characterization, (including monoclonal antibody analysis). and in vitro culture as the methods for diffusion chamber, soft agar, and Dexter type cultures and culture conditions are refined.