LARGE-SCALE PROCESSING OF BIOLOGICAL MATERIALS

试点工厂(生物技术)单位结合了几种不同类型的 活动。它负责大规模生产细菌， 哺乳动物细胞和各种来源的生物活性化合物。 在开展这项活动的同时，它还在开展流程开发工作 与这些准备工作相关，以便能够执行它们 效率很高。此外，该单位正在进行的研究工作没有 必须与当前项目相关联，但工作需要长期进行 对单位业绩的影响。 在过去的一年里，该部队开展了120次不同规模的 制剂，包括微生物生长量从10到300 升，哺乳动物细胞体积增长高达50升，并加工 各种生物材料。 在生长优化方面做了大量的开发工作 重组大肠杆菌和其他微生物，使用补料批次 100g/L湿重的富氧空气工艺及系统 生物质。各种产品都需要这种细菌，特别是 细菌毒素。

The Pilot Plant (Biotechnology) Unit combines several different types of activities. It is responsible for the large-scale production of bacteria, mammalian cells and biologically active compounds from various sources. Parallel to this activity, it is conducting process development work associated with these preparations in order to be able to execute them efficiently. In addition, the unit is carrying on research work not necessarily associated with a current project, but work that has long-term implications for the unit's performance. During the last year, the unit carried out 120 different large-scale preparations, including micro-organism growth in volumes from 10 to 300 liters, mammalian cell growth up to 50 liter volumes and processing of various biological materials. Development work was done in the growth optimization of various recombinant E. coli and other micro-organisms, using the fed batch technique and oxygen enriched air system yielding 100 g/l wet weight of biomass. The bacteria were needed for various products, especially bacterial toxins.