MICROCALORIMETER MEASUREMENTS OF DNA-PROTEIN INTERACTIONS

使用各种蛋白质来改变人类免疫系统的反应是 对器官移植、艾滋病和化疗领域的浓厚兴趣。 在NCI，已经为此目的开发了几种新药。在… 目前，这些药物仅有少量可用(约1 Ml)。通过将这些药物与精心构建的DNA分子和 通过测量热焓，可以确定药物与DNA之间的键相互作用。 NCI和NIDDK的调查人员咨询了BEIB和LTD关于 一种具有测量能力的钽断流微量热计 在80微升样品中加热10微焦耳或更小。第一 实验表明，我们可以在5微焦耳的范围内测量热量 标准误差小于0.5微焦耳。初步结果显示 热焓很低，这表明结合几乎是 独一无二的由信息量驱动。

The use of various proteins to modify the human immune system response is of intense interest in the areas of organ transplants, AIDS, and chemotherapy. At the NCI, several new drugs have been developed for this purpose.. At present, these drugs are available only in small amounts (approximately 1 ml). By mixing these drugs with carefully constructed molecules ' of DNA and measuring the enthalpy, the drug-DNA bond interactions can be determined. Investigators at the NCI and NIDDK consulted BEIB and LTD in regards to tantalum stopped flow microcalorimeter which has the capability of measuring heats of 10 microjoules or less in 80 microliter samples. The first experiments showed that we could measure heats in the range of 5 microjoules with a standard error of less than 0.5 microjoules. Preliminary results show that the enthalpy is very low which indicates that the binding is almost exclusively entropy driven.