HIGH SENSITIVITY PROTEIN ISOLATION/ANALYTICAL SYSTEM

建议购买一套指定票据，以供 猪瘟病毒的分离、测序及成分分析 蛋白质/多肽。该设备包括在线PTH 现有470A气相测序仪的分析/数据系统，高 高灵敏度氨基酸分析仪，微孔高效液相色谱法测定蛋白质/ 多肽的分离和现有多肽的更新 寡核苷酸合成器。这些附加项目将启用 蛋白质/核酸分析实验室(P/NAAL)提供 向多个用户提供增强的功能，并使他们能够 继续进行目前正在进行的广泛研究。这个 这项要求的特别重点是提供一个平衡和 一套协调的分析工具，将使三个 分离、氨基酸分析和序列分析的成分 互为补充。如果任何一个领域是薄弱的，那么就加强 其他领域的能力都被浪费了。需要的项目 P/NAAL主要调查员提供的支助包括研究 苹果酸脱氢酶的功能、序列分析 猪延胡索酶，牛精浆的支持亚单位， 豚鼠前列腺和响尾蛇神经生长因子及其分离， 甲硫氨酸氨基肽酶和N-氨基转移酶的特性及克隆 乙酰转移酶。密歇根大学的其他研究人员 加利福尼亚-欧文需要P/NAAL支持各种不同的 项目。特别是，涉及分析蛋白酶的项目 连接素、细胞色素p-450‘S、蛋白羧甲基转移酶和 描述了受精蛋白的结合。此外, 有相当数量的次要用户具有同样广泛的 兴趣多种多样。这两个主要项目的气息 而较小的用户强烈强调了 现代蛋白质/核酸分析及其集中化的价值 提供此类服务的实验室。

It is proposed to purchase a set of specified instruments for the isolation, sequence and compositional analysis of proteins/peptides. This equipment includes an online PTH analysis/data system for an existing 470A Gas Phase Sequencer, high sensitivity amino acid analyzer, microbore HPLC for protein/ peptide isolation and updates for the existing peptide and oligonucleotide synthesizers. These additional items will enable the Protein/Nucleic Acid Analysis Laboratory (P/NAAL) to provide enhanced capabilities to its multiple users and enable them to continue with the broad range of research currently underway. The particular focus of this request is to provide a balanced and coordinated set of analytical tools that will enable the three components of isolation, amino acid analysis and sequence analysis to be complementary. If any one area is weak, then enhanced capabilities in the other areas are wasted. Projects requiring P/NAAL support from the principal investigators include studies on the function of malate dehydrogenases, the sequence analyses of porcine fumarase, the supporting subunits of bovine seminal plasma, guinea pig prostate and rattlesnake NGF's and the isolation, characterization and cloning of methionine aminopeptidase and N- acetyl transferase. Other investigators at the University of California-Irvine require P/NAAL support for a diverse range of projects. In particular, projects involving analyses of protease nexins, cytochrome p-450's, protein carboxymethyltransferases, and the fertilization proteins binding, are described. In addition, there are considerable number of minor users with an equally broad variety of interests. The breath of these projects from both major and minor users strongly underscores the many applications of modern protein/nucleic analyses and the value of a centralized laboratory for providing such services.