DATA COLLECTION SYSTEM FOR SMALL PROTEIN CRYSTALS

即使它的仪器和方法有很多进步，X射线 衍射仍然需要大的蛋白质晶体，这是非常 很难弄到。有很多很多有结构的蛋白质 因获得水晶而被放弃的项目 小的。在这个项目中，我们要求购买现成的资金 设备，以便建立高速数据采集系统 仍然可以从这些小晶体中按顺序收集良好的数据 来解决这些蛋白质的结构问题。该系统将使用 具有最高亮度源能力的专用X射线发生器 在非常小的焦距(1 Mm X 1 Mm)下输出3.5千瓦的功率。用于X光检查 检测我们计划使用中提供的多线区域检测器 我们的实验室。 一旦完成，这个针对小蛋白囊泡的Mark IV系统将 被安置在大学里。加州大学圣地亚哥分校 蛋白质结晶学。任何蛋白质晶体学基团都可以 请求使用它的时间。然而，我们已经概述了这项研究 6个可能的研究小组的项目。概述的研究 项目涉及到对许多 有趣的酶，包括来自艾滋病毒的酶。

Even with many advances in its instrumentation and method, X-ray diffraction still requires large protein crystals which are very difficult to get. There are many, many proteins structural projects which are abandoned because the crystals obtained are small. In this project, we ask for funds to buy, off the shelf equipment, in order to set-up a high speed data collection system that can still collect good data from these small crystals in order to solve the structure of these proteins. This system will use a special X-ray generator with the highest brilliance source capable of a 3.5 Kw power at a very small focus (.1 mm x .lmm). For X-ray detection we plan to use the multiwire area detectors available in our laboratory. Once finished, this Mark IV System for small protein cystals will be placed in the Univ. of California, San Diego Resource for Protein Crystallography. Any protein crystallography group can request time to use it. However, we have outlined the research projects of 6 possible research groups. The outlined research projects involve the three-Dimensional structure studies of many interesting enzymes including the enzymes from the A.I.D.S. virus.