CELLULAR POLYPEPTIDES ASSOCIATED WITH METASTASIS OF RAT MAMMARY TUMOR CELLS

该项目的发起是为了识别那些 具体涉及作为第一步的转移过程 对它们的提纯和鉴定。利用 从其衍生的转移和非转移细胞 肿瘤细胞的亲代群体是这个项目的基础， 我们已经确认TMT-081-ms细胞 同基因大鼠的转移，而TMT-081-nm没有 同基因大鼠的转移。用放射性标记对这些细胞进行标记 14C氨基酸揭示了几种定性和 它们多肽模式的数量差异。最多的 仅出现在转移瘤中的强烈标记的斑点 细胞(MW/PI)为67/5.5和50/4.5，信号最强 仅在未转移细胞中出现46/6.7和 38/6.1。当32P被用来放射性标记这些细胞时， 所产生的多肽模式与 用~(14)C氨基酸标记获得的。多个 ~(14)C上未观察到~(32)P标记的多肽 放射自显影。又有几个定性的和 从视觉上可以观察到的数量差异 转移瘤与转移瘤之间放射自显影的检查 非转移性细胞系。最贴有标签的地点 仅在转移细胞中出现者分别为98/4.7和24/4.5。 这些多肽大约是三倍于 转移细胞与非转移细胞的强度比较 分别为14/5.2、15/5.4和12/6.3。40/5.9和40/5.9和 40/5.8在32P的强度上至少增加了三倍 标签与 转移细胞。

This project was initiated to identify those polypeptides that specifically relate to the metastatic process as the first step toward their purification and identification. Utilization of metastasizing and non-metastasizing cells derived from the same parent population of tumor cells is fundamental to this project, and we have confirmed that the TMT-081-ms cells do metastasize in syngeneic rats and that the TMT-081-nm do not metastasize in syngeneic rats. Radiolabelling of these cells with 14C amino acids has revealed several qualitative and quantitative differences in their polypeptide patterns. The most intensely labelled spots that occurred only in the metastatic cells were (MW/pI) 67/5.5 and 50/4.5 and the most intense spots occurring only in the non-metastatic cells were 46/6.7 and 38/6.1. When 32P was used to radiolabel these cells, the resultant polypeptide patterns were markedly different from those obtained with 14C amino acid labelling. A number of the 32P-labeled polypeptides could not be observed on the 14C autoradiograms. There were again several qualitative and quantitative differences that could be observed from visual inspection of the autoradiograms between the metastatic and non-metastatic cells lines. The most intensely labelled spots occurring only in the metastatic cells were 98/4.7 and 24/4.5. Those polypeptides that were approximately threefold greater in intensity in the metastatic cells compared to the non-metastatic were 14/5.2, 15/5.4 and 12/6.3. The polypeptides of 40/5.9 and 40/5.8 were at least threefold greater in the intensity of 32P label compared to the corresponding polypeptides in the metastatic cells.