ETIOLOGY OF ALLERGIC REACTIONS IN PLATELETPHERESIS DONORS

1984年2月，数字地面模型由手工转为自动化 为此目的采集的四种血小板采集技术 自动细胞分离设备。在接下来的3年里，11 在芬沃CS-3000上接受采集术的供者 设备发生了急性超敏反应。九 血小板采集术中发生反应及4例反应 发生在粒细胞分离过程中。目的 这项研究是为了确定导致这些疾病的过敏原 反应。使用皮肤测试，放射过敏吸着剂的组合 试验，嗜碱性粒细胞组胺释放试验，特异性IgE- 介导对环氧乙烷的敏化，环氧乙烷是一种用于灭菌的气体 一次性塑料分离工具包，在所有 血小板移植供者出现反应，但100人中没有一人 非反应性对照。初步数据还表明， 环氧乙烷是导致过敏症的因素 粒细胞分离过程中的反应。据估计，由于 多达1.0%的重复采血捐赠者可能会成为 对环氧乙烷敏感，并出现过敏症状 在捐赠过程中。尽管这些反应都不是真实的- 具有威胁的是，DTM正在继续测试来自 对环氧乙烷的敏化，并推迟敏化的个体 来自随后的CS-3000捐赠。

In February, 1984, the DTM converted from manual to automated platelet collection techniques, acquiring for this purpose four automated cell separation devices. During the next 3 years, 11 donors undergoing apheresis procedures on the Fenwal CS-3000 device experienced acute hypersensitivity reactions. Nine reactions occurred during plateletpheresis and 4 reactions occurred during granulocytapheresis procedures. The purpose of this study was to identify the allergen responsible for these reactions. Using a combination of skin tests, radioallergosorbent tests, and basophil histamine release assays, specific IgE- mediated sensitization to ethylene oxide, a gas used to sterilize the plastic disposable apheresis kits, was found in all plateletpheresis donors experiencing reactions, but in none of 100 non-reacting controls. Preliminary data also suggests that ethylene oxide is the agent responsible for hypersensitivity reactions during granulocytapheresis. It is estimated that as many as 1.0% of all repeat apheresis donors may become sensitized to ethylene oxide and experience allergic symptoms during donation. Although none of these reactions have been life- threatening, the DTM is continuing to test apheresis donors from sensitization to ethylene oxide, and to defer sensitized individuals from subsequent CS-3000 donations.