RESPONSE OF MAMMALIAN CELLS TO CHEMOTHERAPY DRUGS

几种已证实有效的化疗药物，如 蒽环类，博来霉素，烷化剂，新癌抑制素，诺贝尔 金属衍生物、VP-16和低氧放射增敏剂正在被 研究了 解毒机制，细胞的修饰 通过改变细胞内氧化还原状态和氧的反应 敏感细胞和抗性细胞中的代谢是本领域技术人员感兴趣的。 癌症治疗领域，并与我们的研究直接相关。 毒品和毒品生产的有害物种， 细胞对这些物质的反应，以及巯基 含有与代谢、活化和代谢有关的化合物， 抗虫塑料的解毒。 已经证明 通过直接缀合或 从头合成的抑制导致细胞致敏 阿霉素、博莱霉素、顺铂、VP-16、烷化剂和低氧 放射增敏剂。 或者，通过增加谷胱甘肽水平， 提供直接的前体导致保护细胞免受 以上试剂。 通过供应治疗后的细胞拯救 谷胱甘肽直接通过修饰分子，使其成为 膜渗透性正在研究中。 交付的时间 救援人员，救援人员如何与其他 生物化学途径、细胞清除和体内清除是 正在调查

Several chemotherapy agent with proven utility such as anthracyclines, bleomycins, alkylators, neocarcinostatin, nobel metal derivatives, VP-16, and hypoxic radiosensitizers are being studied. The detoxification mechanisms, modification of cellular response by altered intracellular redox status and oxygen metabolism in sensitive and resistant cells are of interest to the area of cancer treatment and directly related to our studies. Deleterious species produced by the antineoplastic drugs and cellular response to these species, as well as sulfhydryl containing compounds as they relate to metabolism, activation, and detoxification of antineoplastics. It has been demonstrated that depletion of glutathione levels either by directly conjugating or inhibition of de novo synthesis results in sensitization of cells by adriamycin, bleomycin, cisplatin, VP-16, alkylators, and hypoxic radiosensitizers. Alternatively, increasing glutathione levels by providing direct precursors results in protection of cells from the above reagents. Rescue of cells after treatment by supplying glutathione directly by modifying the molecule such that it becomes membrane permeable is being studied. Timing of delivery of the rescue agents, how the rescue agents interact with other biochemical pathways, cellular clearance and in vivo clearance are being investigated.