STUDIES ON HBLV, EBV, AND HIV

人嗜B淋巴细胞（人疱疹病毒-6）病毒（HBLV），分离 获得性免疫缺陷综合症（艾滋病）患者， 患有其他恶性肿瘤和淋巴增生的患者 也发现了已知具有B细胞表面标记的疾病， 感染从外周血，骨髓， 脾脏胸腺和扁桃体 HBLV也可以复制和产生 在已建立的B-、T- 和巨核细胞系。 人类并发感染 免疫缺陷病毒（HIV）-1和HBLV的T细胞具有T4 受体表现出增强的细胞病变效应和增强的逆转 HIV的转录酶活性。 这两种病毒都可以在 相同的T4细胞，表明HIV-1和HBLV之间的相互作用， 可以通过加速免疫功能的损害， 破坏靶细胞。 这些发现得到了以下方面的支持： HIV-1阳性无症状个体中HBLV抗体升高 （艾滋病相关综合征（ARC），持续性全身性淋巴病 (PGL)和艾滋病）和HIV-1 DNA的存在，约80%的 艾滋病患者的外周血淋巴细胞。 我们还发现HBLV 可以感染EB病毒（EBV）基因组阳性的B细胞 细胞系或B细胞系转化为ESV阳性，表明 EBV诱导HIV和HBLV共同的受体。 HBLV和EBV 非洲伯基特淋巴瘤（BL）血清中的抗体滴度为 升高：10个BL肿瘤中有7个含有ESV和HBLV DHA。 这 提示HBLV在某些B细胞肿瘤中起辅助因子的作用。 HBLV DNA也在两个Sjogren B细胞肿瘤和一个 大B细胞滤泡性淋巴瘤 这些发现是一致的 与HBLV在某些恶性、淋巴增生性 和免疫抑制性疾病。

Human B-lymphotropic (human herpesvirus-6) virus (HBLV), isolated from acquired immunodeficiency syndrome (AIDS) patients and patients with other malignancies and lymphopro-liferative disorders, known to possess B-cell surface markers, was also found to infect T-cells obtained from peripheral blood, bone marrow, spleen, thymus and tonsils. HBLV could also replicate and produce large quantities of virus or viral antigens in established B-, T- and megakaryocyte cell lines. Coincident infection by human immunodeficiency virus (HIV)-1 and HBLV of T-cells possessing T4 receptors showed enhanced cytopathic effects and enhanced reverse transcriptase activity of HIV. Both viruses could be found in the same T4 cell, suggesting that interaction between HIV-1 and HBLV could enhance impairment of immure function by accelerated destruction of target cells. These findings were supported by higher HBLV antibody in HIV-1-positive asymptomatic individuals (AIDS-related complex (ARC), persistent generalized lymphopathy (PGL) and AIDS) and the presence of HIV-l DNA in about 80% of peripheral lymphocytes from AIDS patients. We also found that HBLV could oriy infect Epstein-Barr virus (EBV)-genome-positive B-cell lines or B-cell lines converted to ESV positivity, suggesting that EBV induces a receptor common for HIV and HBLV. HBLV and EBV antibody titers in African Burkitt's lymphoma (BL) sera were elevated: seven of ten BL tumors contained ESV and HBLV DHA. This suggests that HBLV has a role as a cofactor in some B-ce11 tumors. HBLV DNA was a1so detected in two Sjogren's B-cell tumors and a large B-cell follicular lymphoma. These findings are compatible with the implication of HBLV in some malignant, lymphoproliferative and immunosuppressive disorders.