CELLULAR POLYPEPTIDES ASSOCIATED WITH METASTASIS OF RAT MAMMARY TUMOR CELLS

该项目的启动是为了鉴定那些多肽， 具体涉及作为第一步的转移过程， 进行纯化和鉴定。 利用 转移性和非转移性细胞 肿瘤细胞的亲本群体是这个项目的基础。 我们已经证实，TMT-081-MS细胞确实在肿瘤中转移。 TMT-081-NM细胞在同系大鼠中不转移， 同系大鼠 用14-C氨基放射性标记这些细胞 酸揭示了几个定性和定量的差异 在它们的多肽模式中。 标记最强烈的斑点 在TMT-081-MS细胞中发生的最大值为（MW/pI）67/5.5， 50/4.5，最强烈的斑点只发生在TMT- 081-NM细胞为46/6.7和38/6.1。 当32-P放射性标记被 使用这些细胞，可以观察到许多多肽 14-C标签上找不到的 最强烈的 32-P标记的斑点仅出现在转移细胞中， 98/4.7和24/4.5。 这些多肽大约 在转移性细胞中的强度是 非转移细胞：14/5.2，15/5.4和12/6.3。 的 40/5.9和40/5.8的多肽至少是 32-P标记的强度与相应的 转移细胞中的多肽。 一些亚克隆的 转移性TMT-081-MS细胞来源于转移 Wistar/Furth大鼠。 这些亚克隆具有转移能力 并将用于进一步的实验。

This project was initiated to identify those polypeptides that specifically relate to the metastatic process as the first step toward their purification and identification. Utilization of metastasizing and non-metastasizing cells derived from the same parent population of tumor cells is fundamental to this project. We have confirmed that the TMT-081-MS cells do metastasize in syngeneic rats and that the TMT-081-NM cells do not metastasize in syngeneic rats. Radiolabelling of these cells with 14-C amino acids has revealed several qualitative and quantitative differences in their polypeptide patterns. The most intensely labelled spots that occurred in the TMT-081-MS cells were (MW/pI) 67/5.5 and 50/4.5 and the most intense spots that occurred only in the TMT- 081-NM cells were 46/6.7 and 38/6.1. When a 32-P-radiolabel was used with these cells, a number of polypeptides could be observed that were not located from the 14-C labelling. The most intensely 32-P labelled spots occurring only in the metastatic cells were 98/4.7 and 24/4.5. Those polypeptides that were approximately threefold greater in intensity in the metastatic cells compared to the non-metastatic cell: were 14/5.2, 15/5.4 and 12/6.3. The polypeptides of 40/5.9 and 40/5.8 were at least threefold greater in the intensity of 32-P label compared to the corresponding polypeptides in the metastatic cells. A number of subclones of the metastasizing TMT-081-MS cells have been derived from metastasis in Wistar/Furth rats. These subclones possess metastatic capacity and will be used in further experiments.