DEVELOPMENT OF IN VITRO PROPAGATED F344/N MONONUCLEAR CELL LINK

单核细胞白血病的高背景发病率 在Fischer 344只大鼠(20%到30%)中的MNCL混淆了评估 和对可能的化学处理相关的解释 MNCL的发病率在两年的慢性毒理学和 致癌研究。F344大鼠白血病移植模型 被用来描述这种啮齿动物的生物学特性 白血病和研究肿瘤生物学并确定如何 化学处理会影响疾病的表现。最新进展 体外扩增的F344/N单核白血病细胞的应用 将加强：(1)开发独有的抗病毒单抗 用于疾病诊断和分期的MNCL，(2) 目前可用的细胞表面抗原和受体的使用 已知的细胞化学、形态和细胞生物化学数据 数据与白血病细胞来源和功能的测定 血统，以及(3)使用体外试验来确定毒性 和正在研究的化学品在体内MNCL的致癌性 移植模型。 在体外培养的单核白血病细胞将被开发出来 来自体内移植的来自脾中的白血病细胞 肿瘤来源于F344/N大鼠的Vm- 12(在为期两年的研究结束时进行尸检时隔离) 保持自发的MNCL特征。常规组织 基于已知生长和细胞培养的培养技术 正在使用造血细胞和白血病细胞的要求。

The high background incidence of mononuclear cell leukemia (MNCL) in Fischer 344 rats (20 to 30%) confounds the evaluation and interpretation of possible chemical treatment related incidence of MNCL in two-year chronic toxicology and carcinogenesis studies. A F344 rat leukemia transplant model has been developed to characterize the biology of this rodent leukemia and to study the tumor biology and determine how chemical treatment effects disease expression. The development and use of in vitro propagated F344/N mononuclear leukemic cells will enhance: (1) development of monoclonal antibodies unique to MNCL for diagnostic purposes and staging of the disease, (2) the use of currently available rate cell surface antigen and receptor data to known cytochemical, morphological and cell biochemistry data and the determination of leukemic cell origin and functional lineage, and (3) the use of in vitro tests to determine the toxicity and carcinogenicity of chemicals under study in the in vivo MNCL transplant model. In vitro propagated mononuclear leukemic cells will be developed from leukemic cells arising in the spleen from in vivo transplanted tumors derived (and periodically rederived) from F344/N rat VM- 12 (isolated at necropsy at the end of a two-year study) that maintain spontaneous MNCL characteristics. Conventional tissue culture techniques based on the known growth and cell culture requirements of hematopoietic and leukemic cells are being used.