RESOURCES FOR PROTEIN CRYSTALLOGRAPHY

建立真正的国家蛋白质资源需要资金 结晶学。该资源将容纳两个高速数据收集 将提供给蛋白质晶体学小组的系统 美国。第一个系统目前由一个标准组成， 固定源X射线发生器和一个多丝面X射线探测器可以 已经证明，收集数据的速度大约是标准的10倍 衍射仪，可以有效地采集蛋白质晶体的数据 单位晶胞尺寸高达约120埃。第二个系统 由旋转的阳极X射线源和多达6个或更多的区域组成 探测器的速度将至少比标准探测器快100倍 衍射仪，将能够有效地收集蛋白质的数据 晶胞尺寸可达300到400埃的晶体。这 资源将由一个由知名人士组成的顾问委员会指导 来自美国所有地区的蛋白质结晶学家和生物化学家 各州。这个委员会将提供科学的指导，建立 资源准则并确保所有用户公平访问资源 组。核心研究也将是资源的一项重要活动 因为它的目标之一是继续开发更好的区域探测器和 收集蛋白质结晶学数据的更好方法。第三 资源的重要活动是训练其他蛋白质 结晶学家关于数据收集的新方法，因此每个 两年内，资源工作人员将就此设立讲习班和研讨会 主题。保护结晶学家也被鼓励花很短的时间 在资源中心工作一段时间(大约一个月)，以便获得一些 亲身体验。

Funds are requested to set up a truly national Resource for protein crystallography. This Resource will house two high speed data collection systems which will be available to protein crystallography groups in the United States. The first system which presently consists of a standard, fixed-source X-ray generator and one multiwire area X-ray detector can, as has been demonstrated, collect data about 10 times faster than a standard diffractometer and can collect data efficiently for protein crystals with unit cell dimensions up to about 120 Angstroms. The second system consisting of a rotating anode X-ray source and up to 6 or more area detectors will be at least one hundred times faster than a standard diffractometer and will be able to collect data efficiently for protein crystals with unit cell dimensions up to 300 to 400 Angstroms. This resource will be directed by an advisory board composed of well known protein crystallographers and biochemists from all regions of the United States. This board will provide scientific direction, establish the Resource guidelines and assure equitable access to the Resource by all user groups. Core researches wil also be an improtant activity of the resource since one of its aims is to continue to develop better area detectors and better methods to collect protein crystallographic data. The third important activity of the resource is the training of other protein crystallographers on the new methods of data collection, therefore every two years the resource staff will set up workshops and seminars on this subject. Protection crystallographers are also encouraged to spend a short period of time (about one month) at the resource in order to get some hand-on experience.