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REGULATION OF GENE ACTIVITY

REGULATION OF GENE ACTIVITY
基因活性的调节
批准号：
3962990
负责人：
I PASTAN
金额：
$0.0万
依托单位：
DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3962990
关键词：
RNA Rous sarcoma virus epidermal growth factor gene expression genetic library genetic manipulation genetic transcription genetic translation molecular biology molecular cloning mutant natural gene amplification nucleic acid sequence oncogenes oncoproteins squamous cell carcinoma tissue /cell culture

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中文摘要
翻译
EGF受体基因的启动子区含有SP1结合位点 可能调控其表达。 一些其他可能的 在核酸外切酶保护测定中已经检测到调节位点。 一 构建了EGF受体基因的全长cDNA克隆， 用来制造有义和反义RNA。 正义RNA被翻译成 EGFR蛋白。 反义RNA特异性地抑制这种翻译。 已经分离出具有低水平EGF受体的KB细胞的突变体， 正在被定性。
英文摘要
The promoter region of the EGF receptor gene contains SP1 binding sites which probably regulate its expression. A number of other possible regulatory sites have been detected in exonuclease protection assays. A full length cDNA clone of the EGF receptor gene has been constructed and used to make sense and antisense RNA. The sense RNA is translated into EGFR protein. Antisense RNA specifically inhibits this translation. Mutants of KB cells with low levels of EGF receptor have been isolated and are being characterized.
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会议论文
GENETIC ANALYSIS OF THE MULTIDRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
MONOCLONAL ANTIBODIES TO CANCER CELLS
GENETIC ANALYSIS OF THE MULTIDRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
REGULATION OF GENE ACTIVITY
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