ELEMENTAL IMAGING OF ALVEOLAR MACROPHAGES

用光学显微镜观察肺泡内的屈光颗粒 从表现出临床症状的患者身上灌洗巨噬细胞 石棉肺、矽肺和矿工肺气肿。然而，元素 这些颗粒在细胞超微结构中的组成和位置 巨噬细胞不能用光镜观察。元素x射线图像获得于 大面积风干巨噬细胞的Cameca MBX微探针 使研究人员能够将颗粒组成与疾病联系起来。 巨噬细胞超薄切片的高分辨率图像 日立H700H分析电子获得的EPON包埋制剂 利用亮场(BF)、能量色散X射线(EDS)和 电子能量损失(EELS)信号揭示了这两种元素 颗粒的组成和超微结构位置。

Refractile particles are observed by light microscopy (LM) in alveolar lavaged macrophages from patients exhibiting clinical symptoms of asbestosis, silicosis and coal-miner's emphysema. However the elemental composition and location of these particles in the ultrastructure of the macrophages cannot be determined by LM. Elemental x-ray images obtained in the CAMECA MBX microprobe from large fields of whole air-dried macrophages have enabled investigators to correlate particle composition with disease. High resolution images of individual macrophages in ultrathin sections of epon embeded preparations obtained in the Hitachi H700H analytical electron microscope utilizing bright field (BF), energy dispersive x-ray (EDS) and electron energy loss (EELS) signals have revealed both elemental composition and ultrastructural location of the particles.