EFFECTS OF GAMMA-IRRADIATION ON CELLS AND THEIR CONSTITUENTS

可见光的电离和紫外线辐射的影响，以及 超声波对细胞及其成分的作用正在研究中。 酞菁类化合物是一类在水溶液中具有最大吸收的氮杂卟啉 600-700 nm区域，是杀灭哺乳动物的有效光敏剂 细胞，并可能成为替代血卟啉衍生物的候选细胞 肿瘤的光动力疗法(PDT)。量子产额的比较 用于在615 nm处从各种不同的 酞菁类化合物及其对培养哺乳动物细胞的细胞毒性 表明超氧化物不是致命性物质。在一种新的光化学中 反应，由多肽和羧酸形成的脱羧基 酸在313 nm处的光解 金属酞菁化合物的自旋捕捉和电子自旋鉴定 共鸣。这些反应可能涉及到不受欢迎的 光动力疗法后日光所致的光毒性。这个 超氧化物和脱羧基的光化学形成 通过来自抗肿瘤药物卡波昆的可见光(404-615 nm)， 丝裂霉素C和强的松果素被研究是因为 把化疗和光疗结合起来。继续我们对其影响的研究 连续波和脉冲1兆赫(MHz)超声在水中的应用 解决方案，改变脉冲重复频率对瞬变的影响 考察了空化和对羟基自由基生成的影响。这个 与内部自旋捕获氧自由基相关的障碍 对细胞的研究结果表明，初级自由基反应 然而，5，5-二甲基-1-吡咯啉-N-氧化物自旋 与羟基自由基的加合物在内部迅速腐烂 手机。

The effects of ionizing, and ultraviolet radiation, of visible light, and of ultrasound on cells and their constiuents are being studied. Phthalocyanines, a class of Azaporphyrins with maximum absorption in the 600-700 nm region, are efficient photosensitizers for killing of mammalian cells and may be candidates to replace hematoporphyrin derivatives in photodynamic therapy (PDT) of tumors. A comparison of the quantum yields for superoxide radial photogeneration at 615 nm from a variety of Phthalocyanines with their cytotoxicity to cultured mammalian cells suggests that superoxide is not the lethal agent. In a new photochemical reaction, the decarboxylation radicals formed from peptides and carboxylic acids by photolysis at 313 nm in the presence of various Metallophthalocyanines were identified by spin trapping and electron spin resonance. These reactions might be involved in the undesirable phototoxicity induced by sunlight following photodynamic therapy. The photochemical formation of superoxide and of decarboxylatedpeptide radicals by visible light (404-615 nm) from the antitumor agents Carboquone, Mitomycin C and Strptonigrin were investigated because of the possibility of combining chemo with phototherapy. Continuing our studies on the effect of continuous wave and pulsed 1 Megahertz ((MHz) ultrasound on aqueous solution, the effect of varying the pulse repetition frequency on transient cavitation and on the formation of hydroxyl radicals was examined. The obstacles associated with spin-trapping oxygen-derived radicals inside cells were studied and the results indicate that the primary radicals react with the spin traps;however, the 5,5-Dimethyl-1-Pyrroline-N-Oxide spin adduct with hydroxyl radicals decays very rapidly in the interior of the cell.