THE ESTABLISHMENT OF CEREBROVASCULAR SMOOTH MUSCLE CULTURE

本研究涉及纯天然的开发和建立， 脑血管平滑肌培养 建立的细胞系 来源于从脑中获得的微血管的解离细胞， 机械分散和过滤技术。 培养细胞具有组织化学和超微结构特征 平滑肌细胞的特征。 它们是：一个卵形体 核有1到4个核仁和一个颗粒状的，稍嗜碱性的 核周细胞质在整个细胞质中成束排列， 特别是邻近细胞的相对细胞膜。 鉴于这些观察结果，培养的脑血管平滑肌 细胞为研究其功能提供了一种新的模型系统， 与脑血流、血压和整体功能有关 对血脑屏障功能的影响

This study was concerned with the development and establishment of pure cerebrovascular smooth muscle culture. The established cell line originates from dissociated cells of microvessels obtained from brains of rats by mechanical dispersion and filtration technique. The cultured cells display histochemical and ultrastructural features characteristic of smooth muscle cells. They are as follows: an ovoid nucleus with one to four nucleoli and a granular, slightly basophilic perinuclear cytoplasm arranged in bundles throughout the cytoplasm, particularly adjacent to the opposing cellular membranes of the cells. In view of these observations, the cultured cerebrovascular smooth muscle cells provide a new model system for studying their function, especially related to the function of cerebral blood flow, blood pressure and overall to the blood-brain barrier function.