B19 PARVOVIRUS AND ADENO-ASSOCIATED VIRUS

我们的实验室对B19进行基础和临床研究 细小病毒，细小病毒科唯一致病的成员 人类。急性感染导致第五种疾病，一种儿童皮疹 以及成人的多关节痛综合征。在患有潜在疾病的患者中 溶血、急性感染导致一过性再生障碍性危象。在……里面 有潜在免疫缺陷的患者，病毒感染持续存在 导致慢性贫血；细小病毒感染是导致贫血的原因之一 艾滋病患者。该病毒对红系祖细胞具有亲和性。 由于它使用红细胞P抗原作为细胞受体。在.期间 过去一年，一种重组细小病毒疫苗试剂，基于 杆状病毒系统中产生的重组空衣壳及其研制 在我们实验室，已经进入第一阶段的人体志愿者试验。 B19细小病毒空衣壳结构的初步研究 确定了Globoside受体的病毒结合部位。在……里面 分子生物学研究，非结构蛋白，这是 负责病毒复制功能和细胞毒性，有 已被证明与人类ERK-3蛋白激酶相互作用，一种已知的 转录调节因子。我们还研究了两种近缘动物 细小病毒。猴细小病毒的整个序列，它能引起 食蟹猴的贫血已被确定，并与 密切相关的人类病毒。鹅细小病毒已被克隆，其 序列已经被证明出人意料地与腺相关的相似 病毒，细小病毒科的人类成员，依靠病毒 为其繁殖而进行的混合感染。B19细小病毒也被 成功接种食蟹猴并产生瞬时 骨髓衰竭，为某些形式的人类提供了一个动物模型 细小病毒感染。

Our laboratory performs basic and clinical studies of the B19 parvovirus, the only member of the Parvoviridae family pathogenic in humans. Acute infection causes fifth disease, a childhood rash illness and a polyarthralgia syndrome in adults. In patients with underlying hemolysis, acute infection results in transient aplastic crisis. In patients with underlying immunodeficiency, virus infection persists and causes chronic anemia; parvovirus infection is a cause of anemia in patients with AIDS. The virus is tropic for erythroid progenitor cells due to its use of erythrocyte P antigen as a cellular receptor. During the past year, a recombinant parvovirus vaccine reagent, based on recombinant empty capsids produced in a baculovirus system and developed in our laboratory, has entered phase I human volunteer trials. Structural studies of B19 parvovirus empty capsids have tentatively identified the viral binding site for the globoside receptor. In molecular biologic studies, the nonstructural protein, which is responsible for viral replicative functions as well as cytotoxicity, has been shown to interact with the human erk-3 protein kinase, a known transcription regulator. We have also studied two related animal parvoviruses. The entire sequence of simian parvovirus, which causes anemia in cynomolgus monkeys, has been determined and compared to the closely related human virus. Goose parvovirus has been cloned, and its sequence has been shown to be unexpectedly similar to adeno-associated virus, a human member of a Parvoviridae that depends on viral coinfection for its propagation. B19 parvovirus has also been successfully inoculated into cynomolgus monkeys and produced transient bone marrow failure, suggesting an animal model for some forms of human parvovirus infection.