ROLE OF NEUROPEPTIDES AND BIOGENIC AMINES IN NEUROENDOCRINE REGULATION

血管紧张素II受体亚型mRNA定位于选择性脑内 区域通过原位杂交，以确定受体的脑部位 并将这些位点与受体表达的位点相关联 通过配体结合和放射自显影。 血管紧张素II受体亚型 mRNA在发育早期（E12）在大鼠外周组织中表达。 一种新的“非典型”血管紧张素II受体亚型的特点， 它的表达被发现在沙鼠海马中减少， 脑缺血 一个AT1样受体被克隆并测序， 沙鼠 该受体与大鼠AT1A具有高度的序列同源性 受体，并且可能对应于沙鼠海马受体。 CGP 42112对巨噬细胞新型非血管紧张素受体的调节作用 增强巨噬细胞与纤维连接蛋白的粘附，表明 这是一种功能性的重要受体，在巨噬细胞中起作用， 功能 褪黑激素给药被发现减少脑血流量， 以选择性地影响腺苷酸环化酶活性。

Angiotensin II receptor subtype mRNA was localized to selective brain areas by in situ hybridization, to determine the brain sites for receptor formation and to correlate these sites with those of receptor expression by ligand binding and autoradiography. Angiotensin II receptor subtype mRNA was expressed early in development (E12) in peripheral rat tissues. A novel "atypical" angiotensin II receptor subtype was characterized, and its expression was found to be decreased in the gerbil hippocampus after cerebral ischemia. An AT1-like receptor was cloned and sequenced in the gerbil. This receptor has high sequence homology to the rat AT1A receptor, and may correspond to the gerbil hippocampal receptor. Modulation of a novel macrophage non-angiotensin receptor by CGP 42112 was found to enhance macrophage adhesion to fibronectin, indicating that this is a functional, important receptor with a role in macrophage function. Melatonin administration was found to decrease cerebral blood flow, and to selectively affect adenylate cyclase activity.