CORE--REMEDIATION PRODUCT EVALUATION

超级基金的生物和化学修复过程 化学品被认为产生毒性较小的产品。 的 核心的总体目标是测试一些假设， 项目研究者采用的补救策略。 核心的一个方面将是产生补救产品， 超级基金化学品 这将包括但不限于， 通过还原脱氯生产样品， 生产科产生的多氯联苯 核心的 对多氯联苯的关注是由于其全球性的 分布、环境持久性以及各种 毒性作用被归因于它们。 还原脱氯是 一种新的和重要的多氯联苯厌氧微生物转化， 被这个核心和其他地方的研究人员广泛研究。 该过程从联苯环中除去氯， 氯化同系物含量越来越少的混合物。 这 转化可能导致生物活性的变化， 所得到的PCB混合物的组成的差异，因为 各同系物的作用机制可能不同， 力量 厌氧脱氯的生物降解产物 提交给核心委员会的多氯联苯和其他补救产品， 使用本领域已知的多种测试来测定生物活性。 项目生物医学研究者的实验室。 这些 测试包括（例如）对细胞间 通讯，中性粒细胞功能，淋巴细胞母细胞生成，P-450 诱导和蛋白激酶C活化。 的活性 将这些测定中的修复产物与以下物质的活性进行比较： 超级基金化学品的创始人 由此产生的信息 测试将有助于修改补救方案，以确保 降低生物活性和不存在有毒副产物。 在 此外，这一努力也是 补救研究和健康危害研究提出了在这方面 计划项目，并将帮助解决超级基金关注的问题。

The processes of biological and chemical remediation of Superfund chemicals are assumed to generate products with less toxic effects. The overall objective of the Core is to test that assumption for some of the remediation strategies employed by Investigators of the Program Project. One aspect of the core will be to generate remediation products of Superfund chemicals. This will include, but not be limited to, production of samples from the reductive dechlorination of polychlorinated biphenyls (PCBs) generated by the Production Section of the Core. Concern about PCBs has arisen because of their global distribution, environmental persistence and the fact that a variety of toxic effects have been ascribed to them. Reductive dechlorination is a novel and important anaerobic microbial transformation of PCBs that has been studied extensively by investigators in this Core and elsewhere. This process removes chlorine from the biphenyl ring resulting in mixtures containing fewer and less chlorinated congeners. This transformation may result in a change in biological activity due to differences in the composition of the resultant PCB mixtures and because individual congeners probably differ in mechanism(s) of action and potency. Biodegradation products from the anaerobic dechlorination of PCBs, and other remediation products submitted to the Core, will be assayed for biological activity using a number of tests available in the laboratories of Biomedical Investigators of the Program Project. These tests include (for example) assays for effects on intercellular communication, neutrophil function, lymphocyte blastogenesis, P-450 induction, and protein kinase C activation. The activity of the remediation products in these assays will be compared to the activity of the starting Superfund chemicals. Information resulting from this testing will be valuable in modifying remediation schemes to ensure reduction of biological activity and absence of toxic byproducts. In addition, this effort represents an important link between the remediation research and the health hazards research proposed within this program project and will aid in resolving issues of Superfund concern.