SELENOPROTEIN P FUNCTION AND REGULATION OF EXPRESSION

硒是一种必需的痕量金属，具有重要的 抗氧化剂在营养中的作用。这些研究的长期目标 是为了了解硒蛋白P在促进神经元存活中的作用 保护神经细胞免受氧化应激的伤害。此应用程序建议 以最近关于新型硒蛋白的发现为基础，并 提出了硒蛋白P的三个具体目标：(1)研究 硒蛋白P在神经元存活和氧化保护中的作用 胁迫，以及硒吸收、储存和利用的机制。这些 研究将使用神经细胞来源的细胞，并用不同的结构进行转基因 对硒蛋白P进行功能结构域的定位和鉴定 在硒蛋白P的相互作用伙伴中，提供保护的能力 从氧化应激出发，分析硒的储存、吸收机制 和利用率。(2)硒蛋白P在重金属保护中的作用 毒性。调查将研究对重金属毒性的保护， 重金属对硒蛋白P表达的调控及其共定位 转基因细胞中重金属和硒的含量。(3)表达和 硒蛋白P在斑马鱼中的作用原位杂交和 斑马鱼对硒蛋白P需要量和功能的测定 将会进行。

Selenium is an essential trace metal which has important antioxidant functions in nutrition. The long range objectives of these studies are to understand the role of selenoprotein P in promoting neuronal survival and protecting neuronal cells from oxidative stress. This application proposes to build upon recent discoveries with regard to the novel selenoprotein and selenoprotein P. Three specific aims are proposed: (1) To investigate the function of selenoprotein P in neuronal survival and protection from oxidative stress, and mechanism of selenium uptake, storage, and utilization. These studies will use cells of neuronal origin, transfected with various constructs of selenoproteins P, to do mapping of the functional domains and identification of interaction partners of selenoprotein P, the ability to provide protection from oxidative stress, and analysis for mechanism of selenium storage, uptake and utilization. (2) The role of selenoprotein P in protection from heavy metal toxicity. Investigations will study protection from heavy metal toxicity, regulation of selenoprotein P expression by heavy metals, and co-localization of heavy metals and selenium within transfected cells. (3) Expression and function of selenoprotein P in zebra fish. In situ hybridization and determination of requirements and function of selenoprotein P in Zebra fish will be conducted.