PROTEIN CRYSTALLOGRAPHY FOR BIOCHEMISTS

蛋白质结构求解是许多重要领域的必不可少的步骤 从分子生物学、酶学到药物设计。蛋白质结晶学 仍然是这一步最重要的方法。一个令人兴奋的新 蛋白质晶体学的发展在于它可以变得如此常规 在蛋白质晶体学家的帮助下， 开始结晶并分解蛋白质结构。这一发展是 因为生物化学家通常有最有趣的 proteins.因此，建立一个蛋白质 晶体学数据收集设施， 经验丰富的蛋白质晶体学家和生物化学家。该设施将 服务于两个目的：一是为许多研究小组提供 最现代的方法和手段来收集数据，另一个将 提供一个机会来加强 晶体学家和生物化学家。这些联系对于 为了避免发表错误的结构。的目的 我们的建议是购买设备来建立这个设施。此数据收集 设施将包括一个MAR成像板探测器，一套大型 聚焦镜、低温装置和在线计算机。该系统将 安装在P.I中已有的RU 200 X射线发生器上 实验室这一数据收集设施将由13个研究机构共享。 组：3个有经验的蛋白质晶体学家组，10个 生物化学家/生物物理学家团体。这些小组的研究活动是 许多倍-从解决蛋白质的结构， 转导信号样cAMP依赖性蛋白激酶 整个病毒的结构。其他有趣的蛋白质， 研究了纤维蛋白原、反应中心、F1 -ATP酶、二氢蝶啶 还原酶，血栓调节蛋白复合物，细菌毒力因子 病原体，不同的转录因子等。另一个非常重要的 该设施的功能将是培养未来的蛋白质 结晶学家该设施将主要由本科生使用 以及研究生和博士后研究员。与活动的耦合 这个设施将是一个蛋白质晶体学课程，将是 由首席研究员教授。另一门大分子课程 建模是由化学和生物化学系提供， 圣地亚哥超级计算机中心（San Diego Supercomputer Center）另外我们 计划每年举办一次为期三天的蛋白质结晶研讨会 非晶体学家的方法。

Protein structure solution is now a required step in many important fields from molecular biology, enzymology to drug design. Protein crystallography is still the most important method for this step. One exciting new development of protein crystallography is that it can be made so routine that a biochemist group with the help of a protein crystallographer could begin to crystallize and solve protein structures. This development is of great significance because biochemists usually have the most interesting proteins. It is, therefore, very important to set up a Protein Crystallography Data Collection Facility that would be shared by experienced protein crystallographers and biochemist. This facility would serve two purposes: One would be to provide many research groups with the most modern methods and instruments to collect data, and the other would be to provide an opportunity to enhance the contacts between crystallographers and biochemists. These contacts are crucial for biochemists in order to avoid publishing wrong structures. The aim of this proposal is to buy equipment to set up this facility. This data collection facility will include a MAR Imaging Plate detector, a set of large focusing mirrors, a cryo device and an on line computer. This system will be set up on a RU200 X-ray generator already available in the P.I's laboratory. This data collection facility will be shared by 13 research groups: 3 experienced protein crystallographer groups, and 10 biochemist/biophyscist groups. The research activities of these groups are many fold - from solving the structure of proteins involved in transduction signal-like cAMP dependent protein kinase, to the examination of structures of whole viruses. Other interesting proteins that will be studied are fibrinogens, Reaction Center, F1 -ATPase, dihydropteridine reductase, thrombomodulin complexes, virulence factors of bacterial pathogens, different transcription factors etc. Another very important function of this facility will be the training of future protein crystallographers. This facility will be used primarily by undergraduate and graduate students and postdoctoral fellows. Coupling to the activities of this facility will be a course in Protein Crystallography that will be taught by the Principal Investigator. Another course on Macromolecular Modeling is being offered by the Chemistry & Biochemistry department in collaboration with the San Diego Supercomputer Center. In addition, we plan to offer, every year, a three-day workshop on protein crystallization methods for noncrystallographers.