P02 HISTOGRAPH 6650

这项提议要求购买埃彭多夫仪器，用于 利用自动化技术测量组织中的P02 特别研制的微型克拉克电极。我们要求这台仪器是为了 几个相互关联的目的。现在，它几乎已经成为强制性的 使用该仪器对组织P02进行主要测量或 用它来比较临床和临床组织中P02的测量结果 用其他方法进行的动物实验，包括常规的 微电极，因为埃彭多夫装置已经迅速成为 这类测量的“黄金标准”。尤其重要的是 关联使用EPR血氧计等新技术进行的测量 技术，以获得额外的完全可信度 新技术的能力；这包括对 通过两种技术采样潜在的不同领域。高度进化的 埃彭多夫装置的构造使其成为克拉克电极 几种测量类型的选择系统，包括：组织 作为肌肉和肝脏，用传统的Clark测量 由于电极的脆性，电极有很大的不同 提示；对于低P02值，如发生在肿瘤和缺血中，因为 其他商业电极的通常尺寸消耗了太多的氧气； 商业乐器独一无二的能力：能够 沿轨道执行高度受控的测量模式，使用 程序化的步骤和内部重新校准。埃彭多夫体系是 唯一可用的具有必要灵敏度的商业工具， 坚固耐用，以及基本组件的可用性。尽管它有 相对较高的价格，它的获得和维护成本会更低 一个自我构建的系统，因为能力的实现 要生产类似的国产氧电极，需要多年的 体验和其他功能的添加，如 电极尖端的可控穿透和坚固的结构 将大大增加这一时间。发展我们自己的同类产品 埃彭多夫将把关键的努力从新的研究转移到 试图复制成熟的技术。在正文中，提案 我们特别指出，埃彭多夫装置的可用性 将极大地促进所有四个主要用户的进步 在这项提议中进行了合作。我们已经包括了足够多的额外费用 组件，使其具有最大的效率和可行性 对该设备的多个用户的有效性。

This proposal requests the purchase of the Eppendorf apparatus for measuring the p02 in tissues using an automated technique based on a specially developed micro Clark electrode. We request this apparatus for several inter-related purposes. It now has become almost mandatory either to use this apparatus for the principal measurements of tissue p02 or to use it to compare measurements of p02 in tissues for both clinical and animal experiments made by other methods, including conventional microelectrodes, because the Eppendorf apparatus has rapidly become the "gold standard" for such measurements. It is especially important to relate measurements made with new technologies such as EPR oximetry techniques in order to have full credibility for the additional capabilities of the new technique; this includes considerations of the potential different domains sampled by two techniques. The highly evolved construction of the Eppendorf apparatus makes it the Clark electrode system of choice for several types of measurements including: tissues such as muscle and liver for which measurements with conventional Clark electrodes are very different because of the fragility of the electrode tips; for low p02 values such as occur in tumors and in ischemia because the usual size of other commercial electrodes consumes too much oxygen; the capability that is unique in commercial instruments: the ability to carry out a highly controlled pattern of measurements along a track, using programmed steps and internal recalibrations. The Eppendorf system is the only available commercial instrument with the requisite sensitivity, ruggedness, and availability of essential components. In spite of its relatively high price, it would be less expensive to obtain and maintain that a self-constructed system because the attainment of the capabilities to produce comparable homemade oxygen electrodes requires many years of experience and the addition of the other capabilities such as the controlled penetration of the electrode tip and the rugged construction would add significantly to that time. Development of our own equivalent "Eppendorf" would divert critical effort away from new research into attempts to reproduce established technology. Within the body the proposal we indicate specifically how the availability of the Eppendorf apparatus will greatly facilitate the progress of all four of the major users who have collaborated in this proposal. We have included sufficient additional components to make it feasible to have maximal efficiency and effectiveness with multiple users of this apparatus.