DVMT OF MASS SPECTROMETRY AS TOOL FOR XRAY CRYSTALLOGRAPHIC STUDIES OF PROTEINS

我们继续开发质谱学(MS)的用途 促进蛋白质的X-射线原子分辨研究 衍射谱和核磁共振谱。我们发现多发性硬化症是巨大的 在这些研究的每个阶段都很有用，包括：1)确定 重组蛋白构建物的纯度和保真度。2) 阐明紧密折叠的结构域以帮助设计 最优的蛋白质结构。3)评估重原子掺入 4)评估共晶体中多组分的化学计量。 进展继续稳定，我们正在做好准备 另一份描述我们新方法的手稿(这将是 结构生物学家的相当大的用处)。

We continue to develop the utility of mass spectrometry (MS) for facilitating atomic resolution studies of proteins by x-ray diffraction and NMR spectroscopy. We have found that MS is enormously useful at every stage of these studies, including:. 1) Determining the purity and fidelity of the recombinant protein constructs . 2) Elucidating tightly folded structural domains to assist in the design of optimal protein constructs. 3) Assessing heavy atom incorporation 4) Assessing the stochiometry of multiple components in co-crystals. Progress continues to be steady and we are getting ready to prepare another manuscript describing our newer methologies (which will be of considerable use to structural biologists).