ROLE OF ANKYRIN B IN CARDIAC DEVELOPMENT & FUNCTION

Ankyrin-B是空间组织IP3和IP3的候选蛋白质 横纹肌中的兰尼定受体。我们已经敲除了这种基因 在小鼠中通过同源重组编码Ankyrin-B，并 现在正在评估它对心脏发育的影响。我们有 在分离的心肌细胞中发现IP3受体错误排序， 而且钙的释放模式是非常不正常的。锚蛋白-B 衰竭的心脏显示出心室质量的减少，尽管 它们的超微结构基本上是正常的。世界银行的目标是 建议定量评估Anankyrin-B敲打的影响 新生儿的体积和显微结构组成 小鼠心脏常规3D和弥散张量磁共振成像 显微镜。

Ankyrin-B is a candidate protein to spatially organize IP3 and ryanodine receptors in striated muscles. We have knocked out the gene encoding ankyrin-B in the mouse by homologous recombination, and are now evaluating its consequences for cardiac development. We have found in isolated cardiomyocytes that the IP3 receptor is mis-sorted, and that patterns of calcium release are grossly abnormal. Ankyrin-B depleted hearts of exhibit reduction in ventricular mass, although their ultra structure is essentially normal. The goals of the proposal are to quantitatively assess the effects of ankyrin-B knock out on the volumetric and microstructural composition of the neonate mouse heart by using both conventional 3D and diffusion tensor MR microscopy.