ADVANCES IN BIOMEDICAL ANALYSIS BY TOF MASS SPECTROMETRY

说明：本研究的主要目标是开发新的 对生物医学分析从业者有价值的技术和程序 基于飞行时间的特殊和新兴能力 质谱（TOFMS），尤其是与电喷雾结合 离子化（ESI）。 这些特殊的品质， 飞行时间质谱仪的发展是每秒产生50多个质谱 并实现近100%的样本利用效率 导致灵敏度可能超过质谱仪的灵敏度 仅监测几个质量值。 对色谱的影响 检测包括显着降低检测限和（通过 全光谱的额外数据）分析分辨率的提高 功率（对干扰的区分）至少为10倍。 的系统 对于滥用药物的体液的快速和灵敏的筛查将 沿着快速液相色谱 和电喷雾电离。 此外，还提供了一种用于优化 特定化合物的ESI条件将基于 这个实验室刚刚开发出的预测模型。 最后，串联飞行时间质谱仪 使用激光光解离和激光辅助的仪器 碰撞诱导解离将用于一个合作的生物医学 研究旨在确定翻译后酰化位点， 蛋白脂质蛋白中脂肪酸周转率。 它还将用于 研究了电喷雾电离产生的单电荷和多电荷离子， 碎片质谱也与ESI模型相关。

DESCRIPTION: The principal goal of this research is to develop new techniques and procedures of value to practitioners of biomedical analysis that are based on the special and emerging capabilities of time-of-flight mass spectrometry (TOFMS), especially in conjunction with electrospray ionization (ESI). The special qualities made possible by recent developments in TOFMS are the generation of over 50 mass spectra per second and the achievement of nearly 100 percent sample utilization efficiency leading to sensitivities that potentially exceed that of mass spectrometers monitoring only a few mass values. The implications for chromatographic detection include significantly lower detection limits and (through the additional data of the full spectrum) an increase in analytical resolving power (discrimination against interference) of at least 10 fold. A system for rapid and sensitive screening of bodily fluids for drugs of abuse will be developed using these capabilities along with fast liquid chromatography and electrospray ionization. In addition, a system for the optimization of conditions for the ESI of particular compounds will be developed based on a predictive model just developed in this laboratory. Finally, a tandem TOFMS instrument using both laser photodissociation and laser-assisted collision-induced dissociation will be used in a collaborative biomedical study aimed at determining the sites of post-translational acylation and the rate of fatty acid turnover in proteolipid protein. It will also be used to study the singly and multiply-charged ions formed by ESI by means of their fragmentation mass spectra relating these also to the ESI model.