CORE--CELL PHYSIOLOGY

细胞生理学核心支持CF研究中心，提供 获得视频增强光学显微镜和上皮细胞 电生理学，不仅是直接参与CF的研究人员 研究，而且，其他人在肺部研究或上皮细胞研究工作， 电解质运输，因为这些领域是相关的，也有相关性 CF患者。 成像设备包括两个完整的显微镜 图像采集、分析软件、数据存储和 维持活细胞的适当设备（受控环境 和温度）加上一个额外的分析站：1）一个直立的蔡司 ACM显微镜，配备了落射荧光，增强CCD 相机和Universal Imaging的1/FL PC软件。 2)倒置的蔡司 Axiovert 35显微镜，带自动滤光轮、载物台和焦点， 冷却CCD相机和基于Macintosh的成像软件， 检测系统。 电生理学设施包括： 培养箱和电压钳，也配备了受控环境 温度和Millicel ERS探测器 视频增强灯 显微镜主要用于调节的动态实验 用荧光指示染料（Ca， Cl、Na、pH），用于荧光标记的共定位，例如，不同 标记的抗体，并用于三维可视化（3-D 重建）。 电压钳设置（尤辛室）用于 上皮细胞单层的详细电生理分析 过滤器或整个上皮组织（在改良的尤辛室中），和 Millicel ERS探头用于常规测量 跨上皮电导 核心维护和改进 仪器，提供培训和监督用户在使用 技术，还提供质量控制，并作为一个重点， 参与研究者之间的思想交流。 核心费用 使用成像设施的费用，以支付部分 修理和更换陈旧或过时设备的费用 成像设施，并鼓励周到利用这一点， resource. 核心的可用性不仅使研究人员 可以使用技术专业和昂贵的仪器，但允许 让他们集中精力研究感兴趣的生物学问题 而不是仪器的技术方面。 的 CF研究中心研究人员使用本核心， 在过去的五年里， 出版物和赠款支助数量的增加就是证明。

The Cell Physiology Core supports the CF Research Center by providing access to video-enhanced light microscopy and epithelial electrophysiology, not only to researchers directly involved with CF research, but also, to others working on pulmonary research or epithelial electrolyte transport, as these areas are related and also have relevance for CF patients. The imaging facility consists of two complete microscope set-ups with image acquisition, analysis software, data storage, and the appropriate equipment for maintaining live cells (controlled environment and temperature) plus one additional analysis station: 1) an upright Zeiss ACM microscope that is equipped with epifluorescence, an intensified CCD camera and Universal Imaging's 1/FL PC-software. 2) An inverted Zeiss Axiovert 35 microscope with automated filter wheels, stage, and focus, a cooled CCD camera and Macintosh based imaging software from Biological Detection Systems. The electrophysiology facility consists of an incubator and a voltage clamp, also equipped with controlled environment and temperature, and Millicel ERS probes. The video-enhanced light microscopes are used predominantly for dynamic experiments of regulation of intracellular ion concentrations with fluorescent indicator dyes (Ca, Cl, Na, pH), for co-localization of fluorescent tags, e.g., differently labeled antibodies, and for 3-dimensional visualization (3-D reconstruction). The voltage clamp set-up (Ussing chamber) is used for detailed electrophysiological analysis of epithelial cell monolayers on filters or of whole epithelial tissues (in a modified Ussing chamber), and the Millicel ERS probes are used for routine measurements of transepithelial conductance. The Core maintains and improves the instrumentation, provides training and supervision of users in the use of the technologies, also provides quality control and serves as a focus for the exchange of ideas among participating investigators. The Core charges a fee for the use of the imaging facility in order to defray part of the cost of repairing and replacing either worn or outdated equipment of the imaging facility, and to encourage thoughtful utilization of this resource. The availability of the Core not only gives investigators access to technically specialized and expensive instrumentation but allows them to concentrate their efforts on the biological questions of interest rather than on the technical aspects of the instrumentation. The utilization of this Core by the CF Research Center investigators and associates has increased substantially over the past five years as evidenced by the increased number of publications and grant support.