PURCHASE OF A 6 PARAMETER BENCH TOP FLOW CYTOMETER

此共享仪器补助申请用于购买Becton Dickinson FACSCalibur台式流式细胞仪。该仪器将被集成到Englert细胞分析实验室，该实验室是Norris Cotton癌症中心的共享资源，自1981年以来一直为达特茅斯学院和医学院提供流式细胞仪。新仪器将加入FACScan台式流式细胞仪，FACStar Plus细胞分选仪和三个成像系统，位于达特茅斯-希区柯克医学中心Borwell研究大楼的一套房间内。需要额外的台式流式细胞仪是基于两个紧迫的要求：首先，现有的FACScan细胞仪被大量预订，并且他不再能够提供有效研究所需的时间和灵活性。其次，有一个明确的需要，不能满足现有的仪器，四色分析和使用远红激发荧光染料的能力。由于认识到迫切需要购买一台额外的流式细胞仪，达特茅斯医学院研究委员会已确认为购买这台仪器捐款10 000美元。Englert细胞分析实验室在一名主任、一名联合主任和一个顾问委员会的控制下运作，并得到一名流程和一名成像主管的技术支持。一个“主要用户组”已被确定，由11个NIH资助的主要研究者依赖于使用流式细胞仪在恩格勒实验室的研究。1997年，这组研究人员负责92%的FACScan血细胞仪的使用，并预测未来对台式血细胞仪的需求将增加。他们目前持有24个NIH资助的研究赠款，每年直接费用总额为5，109，299美元。

This Shared Instrument Grant application is for funding for the purchase of a Becton Dickinson FACSCalibur bench-top flow cytometer. The instrument will be integrated into the Englert Cell Analysis Laboratory, which is a Shared Resource of the Norris Cotton Cancer Center and which has been providing Dartmouth College and Medical School with flow cytometry instrumentation since 1981. The new instrument will join a FACScan bench-top flow cytometer, a FACStar Plus cell sorter, and three imaging systems in a suite of rooms in the Borwell Research Building at the Dartmouth-Hitchcock Medical Center. The need for an additional bench-top flow cytometer is base don two urgent requirements: First, the existing FACScan cytometer is heavily booked and his no longer able to provide the time and flexibility necessary for efficient research. Second, there is an expressed need that cannot be met on the existing instrumentation for four-color analysis and for the ability to use far-red excitable flurochromes. Because it has recognized the urgent requirement for the purchase of an additional flow cytometer, the Research Committee of Dartmouth Medical School has confirmed a contribution of $10,000 toward the purchase of this instrument. The Englert Cell Analysis Laboratory operates under the control of a Director, a Co-Director, and an Advisory Board, and with the technical support of a flow and an imaging supervisor. A "major user group" has been identified, consisting of eleven NIH-funded Principal Investigators dependent on the use of the flow cytometers in the Englert Laboratory for their research. This group of investigators has been responsible for 92% of the use of the FACScan cytometer in 1997 and predicts increased requirements for a bench-top cytometer in the future. They currently hold 24 NIH-funded research grants totalling $5,109,299 in annual direct costs.