CONNECTIONS OF LIMBIC SYSTEM & HYPOTHALAMUS

我们的目标是生成一个易于访问的MR数据库 一种非人类的定量正常脑发育 与人类关系密切的灵长类动物。我们计划定义特定于年龄的 每个发育阶段的受试者间变异性的正常范围 用于未来脑发育异常研究的测量方法 模仿人类神经解剖学和心理行为疾病。我们 将整合正常大脑的定量成像知识 有生化、组织学和行为数据的发展 在同一只动物身上。 具体目标： 1.使用薄片。高分辨率。冠状3D体积SPGR 非侵入性量化选定的结构变化的序列 神经区域。使用计算机辅助容量分析和 分割技术我们将测量体积并将其可视化 在特定的脑结构中发生的脑形状的变化 发育中的马鞭草Monky。2.使用中固有的信息 磁共振序列上方和从倒置恢复序列进行量化 局部有髓白质。短TR、短TE序列是 对有髓白质敏感，并将用于相同 种群使用与上面1相同的实验设计。3.至 验证具体目标1.和2.通过执行量化 年龄匹配的冷冻肌切除马鞭草脑切片的组织学研究 使用共同的解剖坐标系。 发育中的大脑由不同的处理区域组成，这些区域 在有组织的顺序模式中成熟。增长和发展的模式 鞭毛猴主要大脑区域的发育还没有得到证实 描述。新近发展起来的计算机辅助分割 协议改进了对这两个分子的定量结构分析 人类和灵长类动物的大脑。这些方法依赖于强度 区分和边缘检测的方法假定直接 像素强度与物理衬底的关系。单薄 切片、短回声时间(TE)、短重复时间(TR)、 稳态(SPGR)序列中的梯度回忆捕获 使用高场3.OT磁体可以提供低噪声、高 用于重建各种分辨率的三维数据集 大脑结构的特征。这将允许受试者内部的纵向 脑发育速度和区域模式的评价 由特定神经区域的体积变化决定。

Our goal is to generate an easily accessible MR database of quantitative normal brain development in a species of non-human primate closely related to the human. We plan to define age specific normal ranges of intersubject variability for each developmental measure for use in future studies of abnormal brain development patterned after human neuroanatomic and psychobehavioral disease. We will integrate quantitative imaging knowledge of normal brain development with biochemical, histologic and behavioral data available in the same animal. Specific Aims: 1. Use a thin section. high resolution. coronal 3D volume SPGR sequence to noninvasively quantify structural changes in selected neural areas. Using computer assisted volumetric analysis and segmentation techniques we will measure the volume and visualize changes in brain shape which occur in specific cerebral structures of the developing vervet monky. 2. Use the information Inherent in the MR sequence above and from inversion recovery sequences to quantify regional myelinated white matter. Short TR, short TE sequences are sensitive to myelinated white matter and will be used in the same population using the same experimental design as in 1. above. 3. To validate specific aims 1. and 2. by performing a quantitative histologic study of age-matched cryomycrotomed vervet brain sections using a common anatomic coordinate system. The developing brain is composed of distinct processing regions, which mature in an organized sequential pattern. The pattern of growth and mvelination of major brain regions in the vervet monkey has not been described. Recently developed computer assisted segmentation protocols have improved quantitative structural analysis of both the human and primate brain. These approaches rely on intensity discrimination and edge detection The approaches assume a direct relationship between pixel intensity and physical substrate. A thin slice, short time to echo (TE), short time to repetition (TR), gradient recalled acquisition in the steady state (SPGR) sequence using a high field 3.OT magnet can provide a low noise, high resolution three dimensional data set for reconstruction of a variety of brain structures. This will allow longitudinal intra-subject evaluation of the rate and regional pattern of brain development as determined by volume changes in specific neural areas.