GENETIC MANAGEMENT OF ANIMAL COLONIES

描述(改编自申请人的摘要)：发展 逆转录病毒和B型疱疹病毒SPF猕猴已成为 国内圈养繁殖计划。这些SPF菌落对于 进行艾滋病和与艾滋病有关的研究，并在保护健康方面 照看动物或处理动物组织的人员。这样做的目的是 该项目是开发监测参数的方法，如基因多样性， SPF近亲交配、雄性生殖成功和遗传细分 比目前可能的更经济和更可靠的繁殖群体。 用于高度多态人类的聚合酶链式反应(PCR)引物 将对微卫星基因座进行遗传异质性和孟德尔遗传筛查 几个SPF和其他生物医学上重要的灵长类物种的分离。 四组三个最有效的基因座可在一个 单一反应，仅此一项就满足最低限度的充分性标准 基因管理，将被开发用于基因管理。在… 将对每个多态至少一个等位基因进行测序以确认片段 调整和重新设计与猕猴DNA上的退火点不匹配的引物。 将使用两个最有效的三轨迹多集合来估计 利用专门设计的计算机进行遗传管理的有用参数 软件。基因多样性、近亲交配和遗传细分将 在第二代后代的最早出生时进行评估(即， 那些出生在1997-00年间的人)，以及遗传管理 在此基础上，在没有NIH支持的情况下可持续的计划将是 为每个参与的SPF菌落写的。这笔赠款的时间是 在美国国立卫生研究院对猕猴SPF计划的资助结束后六个月继续 将有足够的时间完成对数据的充分分析，以便设计和 实施自我可持续的遗传管理计划，而不损失 连续性。

DESCRIPTION (Adapted from the applicant's abstract): The development of retrovirus and herpes B virus SPF macaques has become a primary goal in domestic captive breeding programs. These SPF colonies are important for the conduct of AIDS and AIDS-related research, and in protecting the health personnel who care for animals or handle animal tissue. The goal of this project is to develop methods to monitor parameters such as gene diversity, inbreeding, male reproductive success, and genetic subdivision in SPF breeding colonies more economically and reliably than is presently possible. Polymerase chain reaction (PCR) primers for highly polymorphic human microsatellite loci will be screened for genetic heterogeneity and Mendelian segregation in several SPF and other biomedically important primate species. Four sets of three of the most effective loci that are multiplexible in a single reaction, and that alone meet minimum standards of adequacy for genetic management, will be developed for use in genetic management. At least one allele for each polymorphism will be sequenced to confirm fragment sizes and redesign primers that mismatch the annealing sites on macaque DNA. The two most effective three-locus multiple sets will be used to estimate parameters useful for genetic management with specifically designed computer software. Gene diversity, inbreeding and genetic subdivision will be assessed in the earliest born of the second generation of offspring (i.e., those born during 1997-00) of the SPF founders, and a genetic management plan based on this, sustainable in the absence of NIH support, will be written for each participating SPF colony. The timing of this grant to continue six months beyond the end of NIH funding of the macaque SPF program will permit time to complete sufficient analysis of data for designing and implementing a self-sustainable genetic management program without loss of continuity.