ARACHIDONOYL DIACYLGLYCEROL KINASE

几年前，我们发现了一种新形式的二酰基甘油激酶，它优先催化含有酯化花生四烯酸的二酰基甘油分子物种的磷酸化。我们还发现，在成熟的狒狒的睾丸和大脑中存在大量的这种酶。从那时起，我们一直在探索这种酶可能在细胞信号传递过程中发挥作用的可能性，这些过程涉及含肌醇的磷酸甘油三酯。我们已经证明这种酶存在于突触体的制备中，也表明这种酶在猕猴的视网膜中以非常高的浓度存在。后一项发现特别令人感兴趣，因为它提出了磷脂酰肌醇依赖的细胞信号可能在视觉过程中发挥作用的可能性。现在需要的是在视网膜的特定细胞和细胞膜中定位这种酶，我们需要特定的抗体来进行这种免疫化学定位工作。我们已经准备了针对酶序列特定区域的抗体，并在Western blotting实验中使用了这些抗体。但我们还没有成功地将它们用于免疫化学定位工作。这可能是我们将不得不在未来准备单抗的酶。

Several years ago we discovered a new form of diacylglycerol kinase that preferentially catalyses the phosphorylation of diacylglycerol molecular species that contain esterified arachidonic acid. We also showed that high amounts of the enzyme are present in the testis and brain of the mature baboon. Since then we have been exploring the possibility that the enzyme may play a role in cell signaling processes that involve inositol-containing phophoglycerides. We have shown that the enzyme is present in preparations of synaptosomes and have also shown that the enzyme is present in very high concentrations in the macaque retina. This latter finding is of particular interest because it raises the possibility that phosphatidylinositol-dependent cell signaling may play a role in the visual process. What is needed now is to localize the enzyme in specific cells and cell membranes of the retina, and we will need specific antibodies for this immunochemical localization work. We have prepared antibodies directed to specific regions of the enzyme's sequence, and have used these antibodies in Western blotting experiments. But we have not yet succeeded in using them for immunochemical localization work. It may be that we will have to prepare monoclonal antibodies to the enzyme in the future.