COCAINE SENSITIVE DOPAMINE TRANSPORTER SYNTHESIS

多巴胺转运体是可卡因的关键靶点或“受体”。 这就调停了它令人上瘾的特性。因此，理解 控制大脑中多巴胺转运体水平的因素是 对于了解药物成瘾的机制是必不可少的。这 该项目计划开发一种方法来测量合成速率和 多巴胺转运体和5-羟色胺的降解率 传送员也是。5-羟色胺转运体很重要，因为 它是抗抑郁药物的靶点或受体。相当可观 在5-羟色胺转运体的周转方面已经取得了进展 测得其半衰期为3.4天。我们正在进行 确定多巴胺转运体的相同因素。资金来源 NIH/DA11178$141,199 08/01/97-06/30/00出版物BOJA，J.W. Carroll，F.I.，Vaughan，R.A.，Kopjtic，T.和Kuhar，M.J. [125I]RTI-121和其他可卡因类似物在大鼠体内的结合位点 额叶大脑皮层。Synapse 30：9-17,1998。丁永生，莫利纳， P.E.，Fowler，J.S.，Logan，J.，Volkow，N.D.，Kuhar，M.J.和Carroll， 表巴替丁衍生物[18F]NFEP和 [18F]N-甲基-NFEP动力学、尼古丁、效应和毒性。核子。 地中海医院。比奥尔。1999年25：139-148。库哈尔，M.神经递质转运蛋白 不在Synapse里吗？在INABIS‘98-第五届互联网世界上亮相 加拿大麦克马斯特大学生物医学科学大会， 12月7日至16日。特邀研讨会。库哈尔，M.J.，沃恩，R.，乌尔 首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容 多巴胺转运蛋白的显微定位 组织化学。法玛科上将。42：168-170,1998。沃恩，R.A. 胡夫，R.A.，Uhl，G.R.和Kuhar，M.J.多巴胺的磷酸化 转运蛋白和对可卡因的快速适应，在哥伦比亚特区戈尔茨坦， Eisenhofer，G.和McCarty，R.(编辑)儿茶酚胺桥接基础 《科学与临床医学》，学术出版社，圣地亚哥，第页。1042-， 1998年。P51RR00165-38 1998年1月1日-1998年12月31日耶尔克斯地区灵长类 研究中心

The dopamine transporter is a key target or "receptor" for cocaine that mediates its addicting properties. Therefore, understanding the factors which control the levels of dopamine transporter in brain is essential for understanding mechanisms of drug addiction. This project plans to develop a method to measure the synthesis rate and degradation rate of the dopamine transporter, and the serotonin transporter as well. The serotonin transporter is important because it is a target or receptor for antidepressant drugs. Substantial progress has been made in that the serotonin transporter turnover has been measured and its half-life is 3.4 days. We are in the process of determining the same factors for the dopamine transporter. FUNDING NIH / DA11178 $141,199 08/01/97 - 06/30/00 PUBLICATIONS Boja, J.W., Carroll, F.I., Vaughan, R.A., Kopajtic, T. and Kuhar, M.J. Multiple binding sites for [125I]RTI-121 and other cocaine analogs in rat frontal cerebral cortex. Synapse 30:9-17, 1998. Ding, Y.-S., Molina, P.E., Fowler, J.S., Logan, J., Volkow, N.D., Kuhar, M.J. and Carroll, F.I. Comparative studies of epibatidine derivatives [18F]NFEP and [18F]N-methyl-NFEP Kinetics, nicotine, effect, and toxicity. Nucl. Med. Biol. 25:139-148, 1999. Kuhar, M. Neurotransmitter transporters are NOT in the Synapse? Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, December 7-16th. Invited Symposium. Kuhar, M.J., Vaughan, R., Uhl, G., Cerruti, C., Revay, R., Freed, C., Nirenburg, M. and Pickel, V. Localization of dopamine transporter protein by microscopic histochemistry. Adv. Pharmacol. 42:168-170, 1998. Vaughan, R.A., Huff, R.A., Uhl, G.R. and Kuhar, M.J. Phosphorylation of dopamine transporters and rapid adaptation to cocaine, in Goldstein, D.S., Eisenhofer, G., and McCarty, R. (Eds.) Catecholamines Bridging Basic Science with Clinical Medicine, Academic Press, San Diego, pp. 1042-, 1998. P51RR00165-38 1/1/98 - 12/31/98 Yerkes Regional Primate Research Center