TURNOVER AND OXIDATION OF LEG INTRACELLULAR FATTY ACID POOLS

这项研究的目的是确定肌肉如何吸收和燃烧血液中的脂肪。参与者仅限于在研究前两个月保持体重不变的人。本研究的具体目的是确定：1)由基础VO2差异所反映的静息能量消耗差异能否预测瘦弱健康成人平均基础FFA通量的个体间差异；2)性别和种族（高加索人与非洲裔美国人）影响平均VO2和平均基础FFA通量的关系；3)白人和非裔美国成年人肥胖、上体脂肪分布和脂肪细胞大小增加与脂肪细胞大小增加有关，与相对于VO2的平均基础FFA通量增加有关；4)葡萄糖不耐受（NIDDM）高甘油三酯血症患者（肥胖和非肥胖）与葡萄糖耐受、非甘油三酯血症患者（肥胖和非肥胖）相比，肝脏FFA排泄量增加；5)腿部动静脉差异技术预测的细胞内肌肉脂肪酸分数周转率和池大小与同一条腿肌肉活检获得的值相关；6)细胞内脂肪酸分数周转率和肌肉池大小在肥胖、葡萄糖不耐受和瘦、葡萄糖耐受个体中更大。自从我们开始这项研究以来，我们开发了新的方法和技术，这些方法和技术使我们确信不再有理由继续这项研究。我们本可以从这项研究中获得的信息正在收集中。

The purpose of this study is to determine how muscle takes up and burns fat present in the bloodstream. Participation is limited to persons who have maintained a constant body weight for two months prior to the study. The specific aims of this study are to determine whether: 1) Differences in resting energy expenditure, as reflected by differences in basal VO2 predict inter-individual differneces in average basal FFA flux in lean, healthy adults; 2) Gender and race (Caucasian vs African-American) influence the relationship between average VO2 and average basal FFA flux; 3) Obesty, upper body fat distribution and increased fat cell size are associated with increased fat cell size are associated with increased average basal FFA flux relative to VO2 in Caucasian and African-American adults; 4) Hepatic FFA delivery is increased in glucose intolerant (NIDDM) hypertriglyceridemic individuals (obese and non-obese) compared with glucose tolerant, nonmolipedemic individuals (obese and non-obese); 5) Intracellular muscle fatt acid fractional turnover rates and pools sizes predicted from leg arterio-venous difference techniques correlate with values obtained from muscle biopsy of the same leg; 6) Intracellular fatty acid fractional turnover rates and pools sizes in muscle are greater in obese, glucose intolerant and lean, glucose tolerant individuals. Since we began this protocol, we have developed new methods and techniques that have convinced us it is no longer justified to continue with this study. The information we would have gained from this study is being collected.