RENAL BASIS FOR HYPOCITRATURIA

Taegtmeyer博士的团队继续检查动员 常氧和心肌糖原及糖原周转 缺血症。目标是使用13C核磁共振同位素分析来监测 糖原对乙酰辅酶A和能量生成的贡献。这个 基本方案是先耗竭，然后用13C补充糖原 浓缩的糖基单位。从实验中提取的组织 在资源中心进行了分析，并证明了 乳酸、谷氨酸和葡萄糖均能很好地检测出来。然而， 柠檬酸循环周转的时间非常短(3分钟)加上 使用[1-13C]葡萄糖、13C-13C所固有的低浓缩度 没有检测到自旋-自旋耦合。这些实验是 重新设计了[1，6-13C]葡萄糖和更长的循环周期 周转率应产生适合分析的光谱。 (协作期7)报告期：(09/01/97-08/31/98)

Dr. Taegtmeyer's group continues to examine mobilization of glycogen and glycogen turnover during normoxia and myocardial ischemia. The goal is to use 13C NMR isotopomer analysis to monitor the contribution of glycogen to acetyl-CoA and energy generation. The basic protocol uses depletion followed by glycogen repletion with 13C enriched glycosyl units. Tissue extracts from experiments performed in Houston were analyzed at the Resource and demonstrated that lactate, glutamate and glucose could be easily detected. However, with the very brief (3 min) period for citric acid cycle turnover plus the low enrichment inherent in the use of [1-13C] glucose, 13C-13C spin-spin coupling could not be detected. These experiments were redesigned with [1,6-13C] glucose and a longer period for cycle turnover which should yield spectra suitable for analysis. (Collaborative 7) REPORT PERIOD: (09/01/97-08/31/98)