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GAMMA TUBULIN RING COMPLEX STRUCTURE: CENTROMERIC MICROTUBLES:DROSOPHILA EMBRYOS

GAMMA TUBULIN RING COMPLEX STRUCTURE: CENTROMERIC MICROTUBLES:DROSOPHILA EMBRYOS
伽玛微管蛋白环复合体结构：着丝粒微管：果蝇胚胎
批准号：
6120594
负责人：
Yixian Zheng
金额：
$1.18万
依托单位：
BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
项目状态：
已结题
起止时间：
1999-04-01 至 2000-03-31
来源：
https://reporter.nih.gov/project-details/6120594
关键词：
biomedical resource genetics growth /development human tissue nucleic acids plants

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中文摘要
翻译
酵母中的起源识别复合体(ORQ)由6个 亚单位。他们正试图将其晶化以确定其 用X-射线结晶学分析了其结构。与此同时，他们想要 用-STEM测定络合物的化学计量比。 初步结果在一定程度上令人鼓舞，尽管 复杂的似乎：要在碳支撑膜上分崩离析，似乎是 通过短暂的戊二醛固定保存得相当好。这两个 ORC单独和ORC+DNA将被研究
英文摘要
The origin recognition complex (ORQ in yeast is composed of six subunits. They are attemptmg to crystallize it to determme its structure by x-ray crystallography. Meanwhile, they would like to determine the stoichiometry of the complex using-the STEM. Preliminary results have been somewhat encouragmg, in that while the complex seems:to fall apart on the carbon support film, it seems to be fairly well-preserved by a brief glutaraldehyde fixation. Both the ORC alone and the ORC plus DNA will be looked at
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Journal of Genetics and Genomics