TRAINING IN USE OF DMX ELECTRONICS

我们将继续研究其结构和机制。 四氢蝶呤依赖羟基酶。调节和催化作用 酪氨酸、苯丙氨酸和色氨酸羟基酶的结构域 它们都在大肠杆菌中分别表达。结构测定 用X射线衍射法研究酪氨酸的催化结构域 没有或存在底物或底物时的羟基酶 类比将完成。磷酸化对肌动蛋白表达的影响 酪氨酸羟基酶调节域的结构将是 用高场核磁共振方法测定。嵌合蛋白 含有调节性和催化性突变体的 在这些建筑上。的调节和催化性能 突变的蛋白质将被确定。

Studies will continue on the structures and mechanisms of the tetrahydropterin dependent hydroxylases. The regulatory and catalytic domains of tyrosine, phenylalanine, and tryptophan hydroxylase have all been expressed separately in E. coli. The structure determination by X-ray diffraction methods of the catalytic domain of tyrosine hydroxylase in the absence and presence of substrates or substrate analogs will be completed. The effects of phosphorylation on the structure of the regulatory domain of tyrosine hydroxylase will be determined by using high field NMR methods. Chimeric proteins containing regulatory and catalytic mutants will be constructed based on these structures. The regulatory and catalytic properties of the mutant proteins will be determined.