CORE--MICROINJECTION

GT3超家族由异源三聚体和ras- 相关的子类，已成为研究的中心焦点， 了解生长控制和受体信号转导。 在 此外，最近的证据表明，这些蛋白质发挥核心作用， 在多种细胞功能如膜调节中的调节作用， 分泌内吞作用和蛋白质运输。 核心主任已 使这些方法适用于GTP结合蛋白的研究 在细胞功能中。 使用针头显微注射来引入限定量的纯化的 蛋白质、表达质粒和抑制性抗体，以及小的 分子，进入活的体细胞，如成纤维细胞，是一个强大的 帮助分析细胞蛋白质功能作用的工具。 我们已经组装了一个核心设施， 技术提供给PPG应用的研究者， 膜运输的分析。 实验已经开始， 促进技术向这个群体的转移。 因此 该核心的目标是： 1. 为体细胞显微注射提供技术能力 2. 在实验设计和所需的 随后的生物学和生物化学测定， 细胞 3. 培训调查人员使用这种方法。 此外，该小组将努力探索与 通过多种组合分析膜运输 这个团队中的专家

The GTPase superfamily, which is composed of the heterotrimeric and ras- related subclasses, has become a central focus for studies aimed at understanding growth control and receptor signal transduction. In addition, recent evidence suggests that these proteins play central regulatory roles in diverse cell functions such as membrane regulation, secretion endocytosis, and protein trafficking. The Core Director has adapted the use of these approaches to the study of GTP binding proteins in cell function. The use of needle microinjection to introduce defined amounts of purified proteins, expression plasmids, and inhibitory antibodies and small molecules, into living somatic cells such as fibroblasts is a powerful tool to aid in the analysis of the functional roles of cellular proteins. We have assembled a core facility which will give access of this technology to the investigators of this PPG application who work in the analysis of membrane trafficking. Experiments, have already begun to facilitate this transfer of technology to this group. Accordingly, the aims of this core are: 1. to provide the technical capability for somatic cell microinjection 2. to provide aid in the design of experiments and in the required subsequent biological and biochemical assays for effects in the injected cells. 3. to train investigators in the use of this approach. In addition, the group will work to explore novel assays related to the analysis of membrane trafficking through the combination of diverse expertise's that exist within this group.