IDENTIFICATION OF GLYCOPEPTIDE PROCESSING MECHANISMS IN PENICILLIUM

本项目拟研究低分子和高分子的结构 对查氏青霉菌丝体糖肽的重量进行测定， 无论这些聚合物用作前体和/或连接点 或用于肽磷酸半乳甘露聚糖（PPGMS）的锚定物或细胞外 糖水解酶、磷酸酶和蛋白酶，其与 PPGMS。 为了建立前体/产品之间的关系， 菌丝体糖肽和胞外PPGMS，结构 细胞壁、细胞膜和细胞外PPGMS将被 表征了 糖肽之间的结构关系 将通过一维和二维NMR光谱进行研究， 气相色谱/质谱联用分析多肽的氨基酸组成 件. 此外，还将探讨其生物合成关系 通过使用放射性标记的碳水化合物和肽前体， 细胞壁的生物素化，然后分离相应的 来自细胞壁、膜和细胞外部分的糖肽。 建立这些糖肽与细胞壁的锚，或 膜对于理解生理事件是重要的 导致PPGMS、水解酶和潜在的 查氏青霉的过敏原

This project proposes to examine the structures of low and high molecular weight mycelial glycopeptides from Penicillium charlesii and to determine whether these polymers serve as precursors and/or points of attachment or anchors for peptidophosphoglactomannans(PPGMS) or the extracellular glycohydrolases, phosphatases and proteases that are co-released with PPGMS. In order to establish the precursor/product relationships between the mycelial glycopeptides and the extracellular PPGMS, the structures of the cell wall, membrane, and extracellular PPGMS will be characterized. The structural relationships between the glycopeptides will be investigated by one and two dimensional NMR spectroscopy and GC/mass spectrometry in conjunction with amino acid analyses of peptide components. In addition, the biosynthetic relationships will be probed by using radiolabeled carbohydrate and peptide precursors an by biotinylation of cell walls, followed by isolation of the corresponding glycopeptides from the cell wall, membrane, and extracellular fractions. Establishing the anchor of these glycopeptides to the cell wall or membrane is important to the understanding of the physiological events leading to the release of PPGMS, hydrolytic enzymes, and potential allergens from the Penicillium charlesii.