EFFECT OF PLANT PHENOLIC COMPOUNDS ON HUMAN COLON EPITHELIAL CELLS

结肠直肠癌是一种常见且往往致命的癌症。 对这一重要的公共卫生问题进行一级预防是可行的，因为它受到营养和药理因素的显著影响，如膳食脂肪、纤维、微量营养素（即钙和硒）、阿司匹林（阿萨）和其他非甾体类药物。 舒林酸是一种有效的结直肠癌化学预防剂。 槲皮素是一种具有抗炎特性的植物衍生化合物，在临床前研究中抑制结肠癌的发展。 然而，其在预防人类结直肠癌中的有效性尚不清楚。 NSAID调节结肠上皮细胞的更新（诱导细胞静止和凋亡）。 这种作用可能是重要的，他们的疗效作为结肠癌的化学预防剂。 本研究的目的是确定舒林酸和槲皮素对人结肠上皮细胞周转的影响。通过比较和对比这两种化合物对人类结肠细胞的作用，我们希望开始了解NSAID化合物对人类结肠直肠隐窝生理学的影响。 通过这些和未来的研究，我们最终希望预测槲皮素作为结肠癌化学预防剂的潜在效用，并进一步阐明抗炎剂预防结肠癌发生的机制。

Colorectal cancer is a common and often fatal cancer. Primary prevention of this important public health problem is feasible because it is substantially influenced by nutritional and pharmacological factors such as dietary fat, fiber, micronutrients (i.e. calcium and selenium, aspirin (ASA), and other nonsteroidal antiinflammatory drugs. Sulindac is a potent chemopreventive agent for colorectal cancer. Quercetin, a plant-derived compound with anti-inflammatory properties, inhibits colon cancer development in preclinical studies. However, its effectiveness in the prevention of human colorectal cancer is unknown. NSAIDs modulate the turnover (induce cell quiescence and apoptosis) of colonic epithelial cells. This effect may be important for their efficacy as colon cancer chemopreventive agents. The goal of this study is to determine the effects sulindac and quercetin on the turnover of human colonic epithelial cells. By comparing and contrasting the effect of these 2 compounds on colonocytes of humans, we hope to begin to understand the effects of NSAID compounds on the physiology of the colorectal crypts of humans. Through these and future studies we eventually hope to predict the potential utility of quercetin as a colon cancer chemopreventive agent and to shed additional light on the mechanisms by which anti-inflammatory agents prevent colon carcinogenesis.