Core--Microarray facility

受体-配体相互作用和信号转导生物化学的教育是细胞生物学中所有事件的驱动力。这在免疫系统中尤其如此，其特征在于促进细胞分化、增殖、凋亡和效应子功能的活化的许多受体-配体相互作用。因此，信号转导生物化学是理解免疫细胞内稳态的生物学事件的中心。在过去的十年中，免疫细胞信号传导的知识体系已经相当成熟，特别是随着酪氨酸蛋白激酶的中心作用和由此产生的蛋白质-蛋白质相互作用的发现。由于这是一个新兴的领域，用于研究细胞因子或其他免疫细胞激活剂诱导的信号传导事件的技术是相当专业的，可能不容易获得经验不足的实验室没有直接的支持。同样，考虑到从一个受体应用到另一个受体的分析的相似性，人们希望从研究类似系统的人的过去经验中受益。因此，本申请提出了一个核心设施，以容纳专注于免疫细胞信号转导生物化学的专业知识和设备。它将被用作新的研究人员的知识和技术资源，他们发现需要探索各种免疫反应中的信号事件，并将促进专业知识和特定测定的开发和转移到新的环境中。

Receptor-ligand interactions and the education of signal transduction biochemistry are the driving force for all events in cell biology. This is especially true in the immune system, which features numerous receptor- ligand interactions that promote cell-differentiation, proliferation, apoptosis, and activation of effector functions. Accordingly, signal transduction biochemistry is central to understanding the biological events within immune cell homeostasis. The body of knowledge of immune cell signaling has considerably matured over the past ten years, especially with the discovery of the central role for tyrosine protein kinases and the resulting protein-protein interactions. Since it is a nascent field, the techniques used to study signaling events induced by cytokines or other immune cell activators are fairly specialized and perhaps not readily acquired by inexperienced laboratories without direct support. Likewise, given the similarity of assays that apply from one receptor to another, one would hope to benefit from past experience of those studying a like system. Thus, this application proposes a core facility to house expertise and equipment that focuses on immune cell signal transduction biochemistry. It will be used as a knowledge-based and technical resource for new investigators who find a need to explore signaling events in various immune responses and will facilitate development and transfer of expertise and specific assays to a new setting.