CHARACTERIZE STRUCT OF MOLECULAR CHAPERONES CYTOSTRUCTURE OF PROKARYOTIC CELL

我们正在研究一种极端嗜热菌的超微结构 古细菌，柴田硫化叶菌，与假设的分子 这种生物体中的伴侣蛋白形成了丝状 是真核生物的原核版本的细胞结构 细胞骨架 我们的目标是确认丝状的 以前在这些生物体中发现的结构，并阐明它们的 组成;即，以确定它们与分子 伴侣蛋白 策略是尝试优化成像 使用快速冷冻技术，HR-SEM，和 能量过滤显微镜 与纤维相关的蛋白质 通过免疫金或荧光技术鉴定。

We are investigating the ultrastructure of a hyperthermophilic archaeon, Sulfolobus shibatae, with the hypothesis that the molecular chaperonins in this organism form the basis for a filamentous cytostructure that is a prokaryotic version of the eukaryotic cytoskeleton. The goal is to confirm the presence of filamentous structures previously seen in these organisms and to elucidate their composition; i.e., to establish their relationship to molecular chaperonins. Strategy is to attempt to optimize imaging of cytoplasmic structures using quick-freezing techniques, HR-SEM, and energy-filtering microscopy. Proteins associated with filaments will be identified by immun07gold or fluorescence techniques.