APOPTOSIS DIFFERENCES FOR ETHIDIUM BROMIDE BOUND TO RESIDUAL CHROMATIN IN CELLS

荧光寿命分析结合使用 常规的流式细胞术分析监测残留物的变化 人早幼粒白血病细胞株HL-60细胞凋亡过程中染色质的变化 喜树碱、放线菌素、染料木素、H7和伽马射线。数据 表明所有这些代谢抑制物通过不同的方式发挥作用 信号级联，产生减少的凋亡亚群， 但DNA结合的溴化乙锭(EB)的寿命不同。结果 结合常规流程指示寿命分析 流式细胞术，可用于早期检测诱导的细胞凋亡 染色质的变化，也可能提供新的信息 不同的细胞凋亡诱导剂的作用效果。

Fluorescence lifetime analysis was used in combination with conventional flow cytometric analysis to monitor changes in residual chromatin in apoptotic HL-60 cell populations following treatment with camptothecin, cycloheximide, genistein, H7, and gamma radiation. Data show that all of these metabolic inhibitors that act through different signaling cascades, produce apoptotic sub-populations with decreased, but different lifetimes for DNA-bound ethidium bromide (EB). Results indicate that lifetime analysis, in conjunction with conventional flow cytometry, can be useful for early detection of apoptosis-induced chromatin changes and can also potentially provide new information on the effects of different apoptosis inducing agents.