NOVEL METABOLIC INPUTS INTO SYNTHESIS OF THIAMINE IN S TYPHIMURIUM

我们的工作集中在硫胺素的生物合成途径上。 鼠伤寒沙门氏菌。我们目前发现了两种新的新陈代谢 在硫胺素的嘧啶部分的合成中加入 据报道来自从头开始的嘌呤生物合成。我们目前的情况 工作重点是确定涉及到的基因和基因产物 我们有另一种嘧啶生物合成途径(APB) 描述。我们预计这项工作需要从结构上确定 我们从影响硫胺素的突变体中分离出的各种代谢物 综合。这种结构确定的一个步骤是核磁共振 由NMRFAM进行光谱测定。

Our work focuses on the thiamine biosynthetic pathway in Salmonella typhimurium. We have currently found two novel metabolic inputs into the synthesis of the pyrimidine moiety of thiamine which was reportedly derived from de novo purine biosynthesis. Our current work focuses on determining the genes and gene products involved in the alternative pyrimidine biosynthetic pathway (APB) we have described. We foresee this work requiring structural determination of various metabolites we isolate from mutants affecting thiamine synthesis. One step in this structural determination will be the NMR spectral determination carried out by NMRFAM.